小粒野生稻可转化人工染色体文库初步构建及筛选  

The Construction and Screening of Genomic Library Based on Transformable Artificial Chromosome(TAC) Vector in Orazy.Minuta

作　　者：王正华[1] 曹筑荣[1] 曹孟良[2] 

机构地区：[1]国防科技大学计算机学院,湖南长沙410073 [2]国家杂交水稻工程技术研究中心,湖南长沙410125

出　　处：《国防科技大学学报》2008年第1期120-124,共5页Journal of National University of Defense Technology

基　　金：中国博士后基金资助项目(2005037696);湖南省级重点实验室科研资助项目(05FJ4035)

摘　　要：以小粒野生稻核DNA为材料构建了一个可转化人工染色体文库,获得了5000个克隆,平均插入片段约45 kb。稳定性检测结果表明,小粒野生稻基因组DNA能够在TAC载体中稳定存在。基于已克隆抗性基因的保守序列设计探针,利用菌落杂交的方法,对文库进行筛选。结果表明,该文库可以用于抗性基因的筛选。A transformation-competent artificial chromosome （TAC） library was constructed from the genomic DNA of orazy. mimuta. This library is composed of 5,000 clones while the average insertion fragment size is 45 kb. Stability test indicated that genome DNA of Orazy. mimuta was stably existence in the pYLTAC27 carrier. A probe was designed according to the sequences of cloned resistance gene. With the colony hybridization method, the genome library was screened. The result shows that resistance gene can be screened in this library.

关 键 词：可转化人工染色体 基因组文库 小粒野生稻 

分 类 号：Q94-33[生物学—植物学]