HIV-1核蛋白p24在昆虫细胞中的表达  

EXPRESSION OF HIV-1 P24 PROTEIN IN INSECT CELLS AND EVALUATION OF THEIR IMMUNOLOGICAL PROPERTIES

作　　者：谢云[1] 董明[1] 张鹏飞[1] 

机构地区：[1]卫生部北京生物制品研究所

出　　处：《病毒学报》1997年第3期202-207,共6页Chinese Journal of Virology

摘　　要：将完整的ＨＩＶ－１ｐ２４基因克隆到杆状病毒转移质粒中。使用重组转移质粒与野生型杆状病毒（ＡｃＮＰＶ）ＤＮＡ共转染Ｓｆ９昆虫细胞，经筛选获得带有编码ｐ２４基因的重组杆状病毒。重组杆状病毒感染Ｓｆ９细胞后在细胞中表达了ＨＩＶ核蛋白ｐ２４。其重组蛋白的分子量为２４ｋＤ。此重组糖蛋白在免疫荧光，免疫印染和酶联免疫实验中都能被人ＨＩＶ－１阳性血清和单克隆抗体所识别。The full-length cDNA coding HIV-1 p24 were cloned from pZHIV plasmid containing HIV genome and inserted into baculovirus transfer vector.Sf9 insect cells were co-transfected with the recombinant transfer vector plasmids and wild type baculovirus DNA.The recombinant baculovirus containing HIV-1 p24 gene were obtained by limited dilutions.Recombinant HIV-1 p24 with 24kD proteins were expressed by Sf9 cells infected with recombinant virus.The recombinant protein can be recognized by human HIV-1 positive sera and monoclonal antibody against HIV-1 p24 as evidence by Western blot and EIA.

关 键 词：艾滋病毒-1 核蛋白 病毒蛋白p^24 昆虫细胞 研制 

分 类 号：R373.9[医药卫生—病原生物学] R392－33[医药卫生—基础医学]