乙型肝炎病毒核心启动子及其上游顺序对其基因表达的调控  

REGULATION OF HBV GENE EXPRESSION BY CORE PROMOTER AND ITS UPSTREAM SEQUENCE

作　　者：傅磊[1] 吴雪[1] 孔玉英[1] 汪垣[1] 

机构地区：[1]中国科学院上海生物化学研究所

出　　处：《病毒学报》1997年第3期215-223,共9页Chinese Journal of Virology

摘　　要：构建了线性化的、从基本核心启动子（Ｃｐ）开始并有不同长度Ｃｐ上游顺序的、含完整转录单元的ＨＢＶ基因组克隆，以研究Ｃｐ及其上游顺序对ＨＢＶ基因表达的调控及对ＨＢＶ子代ＤＮＡ复制的影响。发现从基本核心启动子Ｃｐ开始的ＨＢＶ转录单元能够有效地起始３．５ｋｂｍＲ－ＮＡ转录。Ｃｐ上游ＥＮＩ顺序对子代ＤＮＡ复制有极强的刺激作用，而ＥＮⅡ更上游顺序对ＥＮⅡ的刺激作用又有所抑制，证明Ｃｐ的转录受其上游顺序的正负双重调控。另外，Ｃｐ上游还存在与ＨＢＶ基因表达的细胞专一性有关的调控顺序。In this study,linearized genome containing the entire HBV transcriptional units were constructed,which initiated from the basic core promoter (Cp) or its upstream regulatory sequences.The transcriptional unit from Cp was sufficient for the transcription,replication and expression of HBV genome.The upstream regulatory sequences of Cp,overlapping with EN Ⅱ,had a strong stimulating effect on the replication of HBV progeny DNA.However,a region upstream EN Ⅱ and Cp (nt 1403-1633) could suppress this activation.It indicated that the transcription and replication of HBV genome depended on both positive and negative regulation.In addition,there were regulatory elements located on upstream of Cp related to the hepatocyte specificity of HBV gene expression.

关 键 词：乙型肝炎病毒 核心启动子 上游调控顺序 表达 

分 类 号：R373.21[医药卫生—病原生物学]