棉铃虫质型多角体病毒 A B 两种类型体外复制特性的比较研究  

IN VITRO REPLICATION OF TWO TYPES OF HELICOVERPA ARMIGERA CYTOPLASMIC POLYHEDROSIS VIRUS

作　　者：沈中建[1] 王久存[1] 乐云仙[1] 苏德明[1] 

机构地区：[1]复旦大学生命科学学院病毒研究室

出　　处：《病毒学报》1997年第3期255-261,共7页Chinese Journal of Virology

摘　　要：分析比较了棉铃虫质型多角体病毒江苏株Ａ、Ｂ两种类型的离体复制特性。ＨａＣＰＶ－Ａ型（ＣＰＶ１４型）病毒可在多种昆虫细胞系中复制，而Ｂ型病毒只能在同源细胞系中增殖；Ａ型病毒的感染率和细胞内游离病毒粒子的滴度均高于Ｂ型病毒；感染细胞持续传代表明，ＨａＣＰＶ－Ａ型病毒感染的ＨＡ－８３１细胞在连续传代７次后，感染率从最初的１０．６％上升到８０％以上，反之，Ｂ型病毒感染的细胞，传代９次后已不能形成典型的多角体。同时，我们还率先成功地建立了ＣＰＶ的空斑测定技术，并初步尝试了混合型ＨａＣＰＶ的分离纯化。Helicoverpa armigera cytoplasmic polyhedrosis virus (Jiangsu isolate) is of two type:type a (designated as CPV type14) and type B.The replication of the two types in different cell lines was studied.Four cell lines were permissive to HaCPV type A replication and large amount of polyhedra were produced,whereas HaCPV type B infected only two homologous cell lines.Serial subcultures of HaCPV-infected cell lines showed that the infectivity of HaCPV type A increased from 10.6% to 82.3% through seven subcultures in HA-831 cells,whereas HaCPV type B infectivity decreased from 8.8% at the first subculture to zero through nine subcultures in HA-831 cells.Also,a plaque assay technique on titration HaCPV was first developed in this paper.

关 键 词：昆虫病毒 棉铃虫 质型多角体病毒 体外复制 

分 类 号：Q965.8[生物学—昆虫学]