DPA对细胞壁缺陷枯草芽胞杆菌耐热性影响的研究  

Study of the Effect on the DPA to Heat Stability of Cell Wall Deficiency Bacillus subtilis

作　　者：王涛[1] 王和[1] 

机构地区：[1]贵阳医学院微生物学教研室,550004

出　　处：《贵州医药》2008年第1期8-10,共3页Guizhou Medical Journal

摘　　要：目的探讨吡啶二羧酸(DPA)对细胞壁缺陷枯草芽胞杆菌耐热性的影响。方法用β-内酰胺抗生素诱导枯草芽胞杆菌(Bacillus subtilis)成为L型并传代培养获得稳定L型纯培养物,采用形态学方法、紫外分光光度法及热稳定性试验检测稳定L型的芽胞、DPA及耐热性。结果在稳定L型不能发现典型形态的芽胞,但可检测到DPA合成代谢活性以致DPA在菌细胞内大量堆积。L型细胞内虽然存在有大量的DPA,但却不能增强其对高温杀菌的抵抗力。结论枯草芽胞杆菌稳定L型丧失形成芽胞的能力,但能合成DPA。然而稳定L型繁殖体细胞内的DPA并不能增强菌细胞对热的耐受能力,DPA增强蛋白质热稳定性的反应需要在芽胞内进行和完成。Objective To probe the effect on the DPA to heat stability of cell wall deficiency Bacillus subfilis. Methods The L-forms of Bacillus subtilis was induced by β-lactam antibiotic and then the pure cultures of stable L-form were isolated and obtained by subculturing on the LEM. The sporulation, biosynthesis of DPA and heat stability of the stable L-form cells were determined by the methods of morphology, photochemistry and heat stability test. Results The spore could not be found in the stable L-form cells, but synthesis of the DPA could be determined in L-form cells so that the DPA were synthesized and accumulated in the stable L-form cells. The DPA could not increase the heat stability of stable L-form cells. Conclusion The mechanism of the DPA synthesis would be promoted following the cell wall deficiency in stable L-form cells. But the DPA did not increase stability of the L-form vegetative cells to heating beside in the spore.

关 键 词：吡啶二羧酸 耐热性 枯草芽胞杆菌 细菌L型 

分 类 号：R37[医药卫生—病原生物学]