抗氧化剂在阻止细胞DNA突变中的交互作用  

INTERACTION OF ANTIOXIDANT IN THE PREVENTION ON MUTATION OF CELL DNA

作　　者：周白云[1] 宗传龙[2] 

机构地区：[1]泰山医学院医学检验系,泰安271000 [2]泰山医学院基础医学部

出　　处：《现代预防医学》2008年第5期937-938,941,共3页Modern Preventive Medicine

摘　　要：[目的]观察大蒜提取物(garlic extract,GE)和维生素E在拮抗烹饪油烟凝集物(condensates of cooking oil fume,COF)的致突变的作用。[方法]在人外周血淋巴细胞培养物中注入一定剂量的GE或一定剂量的维生素E并一定量的COF,观察淋巴细胞的姐妹染色单体交换率(sister chromatid exchanges,SCE)的变化,挑选GE(1:10-4稀释)和维生素E(1:1000稀释)同时注入培养基中(含COF10μl),观察二者对COF的协同抗突变作用。[结果]GE(1:10-4稀释)可显著降低COF处理过的淋巴细胞SCE(P<0.05);维生素E(1:1000稀释)和GE(1:10-4稀释)在降低COF处理过的淋巴细胞SCE方面存在着协同作用(P<0.01)。[结论]GE和维生素E在拮抗COF的致突变性过程中存在着显著的协同效应;一定量的GE亦有一定的拮抗COF的致突变作用。[Objective] To observe antagonistic effects of garlic extract and vitamin E on mutagenicity of condensates of cooking oil fumes （COF）. [Methods] SCEs （sister chromatid exchanges） were observed and compared before and after different amounts of addition of garlic extract or vitamin E to the culture of human blood lymphocytes containing different amounts of COF. GE （1 ： 10^-4 dilution ） and vitamin E （1 ： 1 000 dilution） were injected to the culture of human blood lymphocytes containing COF 10μl. Then compared the changes in SCEs. [Results] Garlic extract （1 ： 10^-4 dilution） could cut down SCEs of lymphocytes （P〈 0.05） ; garlic extract and vitamin E had a very significant synergistic effect on cutting down SCEs of lymphocytes treated by COF （P 〈 0.01 ）. [Conclusion] Garlic extract and vitamin E have a synergistic effect to antagonize the mutagenieity of COF; garlic extract alone can also cut down SCEs of human blood lymphocytes.

关 键 词：抗氧化剂 DNA突变 交互作用 

分 类 号：R284.1[医药卫生—中药学] R339.38[医药卫生—中医学]