树舌多糖GF对HepA瘤细胞N-ras、C-myc基因表达的影响  

Effect of ganoderma appanatum polysaccharides GF on N-ras、C-myc oncogene expressions in HepA tumor cells

作　　者：宋高臣[1] 于英君[2] 

机构地区：[1]牡丹江医学院生物化学与分子生物学教研室,黑龙江省牡丹江市157011 [2]黑龙江中医药大学生物化学与分子生物学教研室,黑龙江省哈尔滨市150040

出　　处：《同济大学学报（医学版）》2008年第1期45-47,52,共4页Journal of Tongji University(Medical Science)

基　　金：黑龙江省科技攻关项目(G00C200902)

摘　　要：目的为探明树舌多糖GF对HepA瘤组织N-ras、C-myc基因表达的影响。方法运用原位杂交法、SP免疫组化染色技术及多媒体彩色病理图文分析系统测定HepA瘤细胞中N-ras、C-myc mRNA及蛋白的表达量。结果树舌多糖组、猪苓多糖组中N-ras、C-myc mRNA及蛋白的表达量均显著低于荷瘤组(P<0.01),树舌多糖组与猪苓多糖组无显著性差异。结论树舌多糖GF可通过降低N-ras、C-myc mRNA及蛋白的表达,而抑制HepA瘤细胞的增殖。Objective To study the effect of ganoderma appanatum polysaccharides GF（GAPSGF） on N-ras, C-myc expression in HepA tumor cells. Methods In situ hybridization（ISH） and immunohistochemical staining were used to determine the effects of GF and PUPS on N-ras,C-myc mRNA and protein expressions in mouse HepA tumor cells. Results GF and PUPS could significantly reduce the expressions of N-ras and C-myc in HepA cells as compared with those in control group. The expressions of N-ras and C-myc in HepA cells had no differences between GAPSGF group and PUPS group. Conclusion GF and PUPS can inhibit the activity of Hep A cells by reducing the expressions of N-ras and C-myc.

关 键 词：树舌多糖GF HEPA瘤细胞 C-MYC基因 N-RAS基因 

分 类 号：R285.5[医药卫生—中药学]