脂联素基因启动子区多态性与肥胖、2型糖尿病的相关性  

Association of SNPs in the proximal promoter region of the APM1 gene with obesity and type 2 diabetes

作　　者：叶枫[1] 何岚[1] 李建宁[1] 董春萍[1] 于杰[2] 

机构地区：[1]西安交通大学医学院第一附属医院内分泌科,陕西西安710061 [2]西安交通大学医学院遗传学与分子生物学系,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2008年第1期74-76,83,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省科学技术研究发展计划资助项目(No.2005K14-G6)

摘　　要：目的探讨中国人群内脂联素基因启动子区-11391G/A、-11377C/G基因多态性以及突变与肥胖、2型糖尿病(T2DM)的相关性。方法选取研究对象共305名,利用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)方法鉴定-11391G/A、-11377C/G基因型。结果①在所选群体中未发现-11391G/A突变;②以基因型分组:-11377 GG基因型体重指数(BMI)值明显高于CC,CG基因型,且具有统计学意义(P<0.05);GG基因型舒张压、收缩压明显高于CC,CG基因型,且与CG基因型差别有统计学意义(P<0.05);CC基因型高密度脂蛋白(HDL)最高且与CG基因型差别有统计学意义(P<0.05)。结论在西安人群内未发现-11391G/A突变;-11377C/G基因多态性与BMI的相关性具有统计学意义;未发现11377C/G基因多态性与2型糖尿病的相关性。Objective To study the correlation of SNPs -11391G/A, -11377C/G with obesity and type 2 diabetes. Methods The PCR-RFLP technique was adopted to test the gene frequencies of adiponectin in 305 people. Results （1） The -11391G/A mutation was not found in our research population; O Subjects with the GG genotype at position -11377 had a significantly higher BMI than those with the GC, CC genotype （P〈0.05）; more over, GG genotype at position -11377 had an increased risk of high systolic blood pressure and diastolic blood pressure compared with GC genotype （P〈0.05）. HDL had a significant difference between CC and CG genotype （P〈0.05）. Conclusion The-11391G/A mutation has not been found in our research population; SNP -11377C/ G may contribute to the genetic risk for BMI but not T2DM.

关 键 词：脂联素 糖尿病 肥胖 基因多态性 

分 类 号：R587.1[医药卫生—内分泌]