TGF-β_1反义寡核苷酸对肾间质细胞的作用  

Effect of Transforming Growth Factor-β_1 Antisense Oligonucleotides on Renal Interstitial Cell

作　　者：崔飞伦[1] 邱镇 陆洪兵[1] 姚震[1] 林大春[1] 

机构地区：[1]江苏省镇江市第一人民医院泌尿外科,江苏镇江212002 [2]解放军359医院泌尿外科,江苏镇江212000

出　　处：《医学临床研究》2008年第2期287-290,共4页Journal of Clinical Research

摘　　要：【目的】探讨转化生长因子β1(TGF-β1)反义寡核苷酸(antisense oligonucleotides,ASODN)在肾纤维化发病机制中的作用。【方法】采用RT-PCR、Western blot检测脂质体介导的TGF-β1ASODN目的基因及蛋白的表达;通过细胞生长试验、流式细胞仪检测TGF-β1ASODN转染前后细胞增殖和凋亡的变化。【结果】转染后可见TGF-β1的基因及蛋白表达明显下调;和对照组相比,可明显抑制成纤维细胞的增殖,诱导其凋亡(P<0.01)。【结论】转化生长因子β1反义寡核苷酸对肾间质成纤维细胞具有明显的抑制作用,转染脂质体介导的TGF-β1ASODN在治疗肾纤维化探讨中可能具有重要意义。[Objective]To investigate the effect of transforming growth factor-β1（TGF-β1）on the proliferation and apoptosis of renal interstitial cell. [Methods] HEK293 cell line was treated with TGF-β1 antisense oligodeoxynucleotides （ASODN） by lipofectamine. The inhibition of proliferation and apoptosis of the cell were studied with cell growth curves, MTT method and flow cytometry. The expression of TGF-β1 was detected by RT-PCR and Western blot respectively. [Results]After transfeetion, the mRNA and protein expression of TGF-β1 in the cells were significantly decreased or negative expression. Compared with the control group, TGF-β1 significantly inhibited cell proliferation and induced cell apoptosis（ P〈0.01）. [Conclusion] TGF-β1 ASODN may significantly inhibit the proliferation and induce apoptosis of renal interstitial cell. These results suggest that infected TGF-β1 ASODN by lipofectamine has an important role in the treatment of renal fibrosis.

关 键 词：肾/细胞学 转化生长因子Β 寡核苷酸类 反义 

分 类 号：R692[医药卫生—泌尿科学]