C市管网末梢水有机提取物对雌性小鼠性激素水平和动情周期的影响  

Effect on Sex Hormone and Estrous Cycle in Female Mice Induced by Organic Extract from Tap Water of City C

作　　者：张建江[1] 舒为群[1] 曹波[1] 陈济安[1] 赵清[1] 邱志群[1] 罗教华[1] 许川[1] 

机构地区：[1]第三军医大学环境卫生学教研室

出　　处：《环境与职业医学》2008年第1期34-36,40,共4页Journal of Environmental and Occupational Medicine

基　　金：国家科技部西部引导项目(编号:2003BA869C);重庆市重大科技专项(编号:CSTC2006AA7003);国家自然科学基金重点项目(编号:30630056)

摘　　要：[目的]观察C市管网末梢水中有机提取物(organic extract,OE)对雌性小鼠性激素水平和动情周期的影响及茶多酚(tea polyphenols,TP)的拮抗效应。[方法]以XAD-2树脂为材料的吸附柱,采用固相萃取技术富集2900L水中的有机污染物并用二甲基亚砜定容。将昆明种雌性小鼠随机分为6组,每组20只,分别给予二甲基亚砜(作为溶剂对照,Ⅰ组)、OE12.5L/kg体重(Ⅱ组)、OE25.0L/kg体重(Ⅲ组)、OE50.0L/kg体重(Ⅳ组)、TP100.0mg/kg体重+OE50.0L/kg体重(Ⅴ组)、TP200.0mg/kg体重+OE50.0L/kg体重(Ⅵ组),连续腹腔注射5d(TP在OE给予前5天开始灌胃直至实验结束),OE染毒后的第15天处死各组中处于动情间期的小鼠,采用电化学发光免疫测试方法测定性激素水平,第30天处死剩余的动物。分别检测染毒后第15天和第30天小鼠肝脏匀浆中的超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)水平。[结果]Ⅳ、Ⅴ及Ⅵ组小鼠血清中的雌二醇水平明显升高,与Ⅰ组相比差异具有统计学意义(P<0.05),而各组血清中的孕酮水平相差未见统计学意义(P>0.05)。在染毒后1～15d,染毒组动物平均每个动情周期的持续时间延长,其中Ⅳ、Ⅴ及Ⅵ组与Ⅰ组比较,差异具有显著的统计学意义(P<0.01)。染毒后第15天时,两个TP组(Ⅴ、Ⅵ)肝脏中GSH-Px含量比IV组增高,其中VI组与IV组差别有统计学意义(P<0.05);而到第30天上述各组间差异无显著性。[结论]水中有机提取物对雌性小鼠表现出一定的模拟雌激素效应及内分泌干扰效应,但茶多酚对这些效应未显示出拮抗作用。[Objective ] To study the effects of organic extract（ OE ）from tap water of city C on sex hormone and estrous cycle in female mice and the antagonism of tea polyphenols. [ Methods ] Organic pollutants were extracted with XAD-2 resin from 2 900 L tap water and then injected intraperitoneally for 5 consecutive days to female mice. The mice that exhibited normal estrous cycles were divided into six groups randomly, treated daily by DMSO（ vehicle control, group Ⅰ ）, OE 12.5 L/kg·bw（ group 11 ）, OE 25.0 L/kg·bw（ group Ⅲ ）, OE 50.0 L/kg·bw（ group Ⅳ ）, tea polyphenols（ TP ） 100 mg/kg·bw ＋ OE 50.0 L/kg.bw（ group Ⅴ ）, TP 200 mg/kg·bw ＋ OE 50.0 L/kg·bw（ group Ⅵ ）. TP was administered by gavage for 5 consecutive days for group Ⅴ and Ⅵ respectively before intraperitoneal injection of OE, and dosing solutions were prepared in fresh distilled water. On the 15th day after treatment, the mice were killed and the levels of sex hormone were determined by Electrochemiluminescence immunoassay （ ECLI ）. At the end of the study, the remaining of the mice was decapitated too. The activities of SOD and GSH-PX in liver were measured. [ Results ] Estradiol levels in blood serum increased in group Ⅳ, Ⅴ and Ⅵ significantly（ P 〈 0.05 ）compared with Group Ⅰ . However, progesterone levels were not altered in treated animals. The length of estrous cycle prolonged with an increase in estrous phases in treated animals at the first period of the study. On the 15th day after treatment, activities of GSH-PX of group Ⅵ significantly（ P 〈 0.05 ）increased compared with Group Ⅳ, while on the 30th day, the results was not exist. [ Conclusion ] The results suggest that the organic pollutants in drinking water bear estrogen-like effects and the effects of environmental endocrine disruptors. TP has little protective effects on these reproductive harmful effects induced by OE.

关 键 词：管网末梢水 有机提取物 雌二醇 孕酮 动情周期 茶多酚 

分 类 号：R114[医药卫生—卫生毒理学]