妊娠期溴氰菊酯暴露对仔鼠神经行为发育及脑Na^＋-K^＋-ATPase活性的影响  

Effects of Deltamethrin on Neurobehavioral Development and the Activity of Brain Na^+-K^+-ATPase in Offsprings of Intoxicated Rats

作　　者：李涛[1] 陈亮[1] 石年[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院环境毒理系；华中科技大学同济医学院公共卫生学院教育部环境与健康重点实验室,湖北武汉430030

出　　处：《环境与职业医学》2008年第1期69-71,共3页Journal of Environmental and Occupational Medicine

摘　　要：[目的]探讨妊娠期溴氰菊酯(deltamethein,DM)暴露对仔鼠脑Na+-K+-ATPase活性及神经行为发育的影响。[方法]确定母鼠妊娠后,随机分为0、3.35及6.70mg/kgDM染毒组,自妊娠1～19d按0.5ml/100g体重隔日一次灌胃染毒,对照组给予相同剂量的色拉油。分别选择5、10、21d龄仔鼠断头处死,测其脑组织Na+-K+-ATPase活性;选择30d龄仔鼠进行主动逃避实验及旷场实验。结果]3.35mg/kg染毒组仅21d龄仔鼠存活率与对照组相比明显降低(P<0.05),6.70[mg/kg染毒组5、10、21d龄仔鼠存活率均低于对照组(P<0.01)。3.35mg/kg染毒组5d龄仔鼠脑组织Na+-K+-ATPase活性无明显变化,10d龄仔鼠活性降低,与对照组相比有显著性差异(P<0.05),21d龄仔鼠未见异常;6.70mg/kg染毒组5、10d龄仔鼠脑组织Na+-K+-ATPase活性显著降低,21d龄未见明显变化。6.70mg/kg染毒组30d龄仔鼠延迟时间为(3.86±1.40)s、被动逃避反应阳性率为24%,与对照组[(1.68±0.70)s、6%]相比,差异有显著性(P<0.05)。3.35和6.70mg/kg染毒组仔鼠旷场实验中行进格子数均明显降低,与对照组的差异均有显著性(P<0.05)。[结论]孕鼠妊娠期接触DM可以抑制仔鼠脑组织Na+-K+-ATPase活性,并对其生长发育产生影响。[ Objective ] To To study the effect of dehamethrin （ DM ）on the neurobehavioral development and the activity of Na^＋-K^＋-ATPase of the filial brains of intoxicated rats. [ Methods ] Impregnate female rats were randomly divided into 3 groups and treated orally with 0, 3.35, and 6.70 mg/kg dehamethrin respectively every other day from pregnant day（ PND ） 1 to 19. Then the activity of brain Na^＋-K^＋-ATPase and the neurobehavioral functions of filial rats were observed. [ Results ] The survival rate in 3.35 mg/kg group was decreased significantly at PDN 21 and that in 6.70 mg/kg group was less than the control group at either PND 5, 10 and 21 （ P 〈 0.01 ）. Na^＋-K^＋-ATPase activity in 3.35 mg/kg group showed no change at PND 5 and 21 compared with the control group while it decresed significantly at PND 10 （ P 〈 0.05 ）. This enzyme activity in 6.70 mg/kg group decreased significantly at PND 5 or 10（ P 〈 0.05 ）while no significant change was found at PND21. The delay time of dark escaping test in the filial rats of 6.70 mg/kg group was（ 3.86 ± 1.40 ）s, and the positive rate of passive escaping response was 24%, while those in control group were（ 1.68 ± 0.70 ）s and 6% respectively. The learning ability and memory function of filial rats in exposed group also decreased （ P 〈 0.05 ）. [ Conclusions ] Exposure to high dose of deltamethrin in pregnant female rats would influence the activity of brain Na^＋-K^＋-ATPase and interfere the neurobehavioral development of their filial rats.

关 键 词：溴氰菊酯 NA^+-K^+-ATP酶 神经行为发育 

分 类 号：R114[医药卫生—卫生毒理学] R994.6[医药卫生—公共卫生与预防医学]