大鼠增龄过程中椎间盘退变指标的研究  

Indexes of intervertebral disc degeneration in rats during the aging process

作　　者：张银刚[1] 刘江涛[1] 王金堂[1] 郭雄[2] 

机构地区：[1]西安交通大学医学院第一附属医院骨科,陕西西安710061 [2]西安交通大学环境与疾病基因教育部重点实验室／环境与地方病研究室,陕西西安710061

出　　处：《南方医科大学学报》2008年第2期169-172,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30400163)~~

摘　　要：目的研究大鼠增龄过程中椎间盘退变的指标。方法同批SD大鼠21只,分别喂养6月(9只)和22月(12只)建立青年大鼠组和老龄大鼠组模型,计算椎间盘退变Miyamoto分级值,体视法分析椎体软骨终板血管芽数量、面积、钙化层和非钙化层厚度,单克隆抗体免疫组化染色测定软骨Ⅱ型和X型胶原的表达。结果老龄组大鼠椎体软骨终板血管芽数量、血管芽相对面积、非钙化层/钙化层比值、钙化层和髓核Ⅱ型胶原含量均低于青年组(P<0.05);老龄组非钙化层X型胶原含量高于青年组(P=0.003);而Miyamoto分级老年组和青年组无统计学差异(P=1.130)。结论终板血管芽相对面积、非钙化层/钙化层比值、Ⅱ型胶原和X型胶原表型表达是评估增龄性椎间盘退变的灵敏指标,而Miyamoto分级不是评估增龄性椎间盘变化的灵敏指标。Objective To study the indexes for evaluating intervertebral disc degeneration in rats during the aging process. Methods Nine SD rats were ＇fed for 6 months and 12 for 22 months as the young and aged groups, respectively. The Miyamoto＇s grade of the rats was calculated, and the quantity and relative area of the vascular buds as well as the thickness of the calcified and non-calcified layers of the cartilage endplate were measured using the stereoscopic method. with monoclonal antibodies was used to determine the expressions of collagens Ⅱ and X in the endplate. Results The quantity and relative area of the vascular buds, non-calcified layer/calcified layer ratio, type Ⅱ collagen expression in the calcified layer and nucleus pulposus of the cartilage endplate were all significantly decreased in the aged rats as compared with those of the youth rats （P〈0.05）, but the collagen X expression in the non-calcified layer was significantly higher in the aged rats （P=-0.003）. No significant difference was found in the Miyamoto＇s grade between the aged and young rats （P=1.130）. Conclusion The relative area of the vascular buds, ,non-calcified layer/calcified layer ratio, phenotypic expressions of collagens Ⅱ and X in the cartilage endplate, but not the Miyamoto＇s grade, are sensitive indexes for evaluating intervertebral disc degeneration in rats during the aging process.

关 键 词：椎间盘退变 增龄 终板血管芽 胶原 Miyamoto分级 

分 类 号：R681.5[医药卫生—骨科学]