携带HBVC基因重组MVA假病毒颗粒的构建及其抗原性鉴定  

Construction and antigenic evaluation of a recombinant MVA virus-like particle expressing HBV C gene

作　　者：栾翔凌[1] 孔维[2] 刘素军[3] 雷厉[4] 胡燕[4] 侯俊[4] 沈宏辉[4] 吴贻琛[4] 游绍莉[4] 貌盼勇[4] 辛绍杰[4] 

机构地区：[1]中国人民解放军军医进修学院,北京100853 [2]吉林大学,吉林长春130023 [3]北京朝阳医院京西院区,北京100043 [4]中国人民解放军302医院,北京100039

出　　处：《南方医科大学学报》2008年第2期252-254,共3页Journal of Southern Medical University

摘　　要：目的构建携带HBVC基因的重组MVA假病毒颗粒并鉴定其抗原性。方法将HBVC基因通过基因重组构建入穿梭载体pSC11,得到质粒pSC11-C,将此质粒转染MVA病毒通过同源重组得到MVA-C。结果利用基因测序,PCR鉴定证实所得假病毒颗粒携带HBVC基因,并且具有良好的抗原性。经过9次传代得到单克隆重组病毒。结论成功构建了携带HBVC基因的重组MVA假病毒颗粒MVA-C,为研究HBV慢性感染的基因治疗提供了新途径。Objective To construct the virus-like parcel expressing hepatitis B virus （HBV） C gene and identify its immtmogencity. Methods HBV C gene was cloned into the shuttle vector pSC11, and the resulted plasmid pSC11-C was transfected into modified vaccinia virus Ankara （MVA）. Results pSC11-C was correctly constructed as verified by sequence analysis and PCR, and the recombinant virus-like parcel possessed good immtmogencity. Coneluslon The MVA-C expressing HBV C gene has been successfully constructed to provide important basis for gene therapy research of chronic HBV infection.

关 键 词：痘苗病毒 肝炎病毒 乙型 基因治疗 

分 类 号：R392[医药卫生—免疫学]