表面增强激光解析电离光谱技术分析人卵泡发育过程中蛋白质组学变化  

Proteomics of follicular fluid in mature human follicles and antral follicles:a comparative study with laser desorption/ionization-time of flight-mass spectrometry

作　　者：李黎[1] 邢福祺[2] 陈士岭[2] 孙玲[2] 李红[2] 

机构地区：[1]成都军区昆明总医院妇产科,云南昆明650032 [2]南方医科大学南方医院妇产科生殖医学中心,广东广州510515

出　　处：《南方医科大学学报》2008年第2期275-278,共4页Journal of Southern Medical University

摘　　要：目的应用蛋白质芯片和质谱技术,建立人成熟卵泡液及窦卵泡液的蛋白质表达指纹图谱,寻找在卵泡生长发育过程中起关键作用的蛋白质。方法采用表面增强激光解析电离飞行时间质谱技术和弱阳离子交换蛋白质芯片检测48例人成熟卵泡液、21例窦卵泡液中的蛋白质变化,使用PBSⅡ-C型蛋白质芯片阅读机读取数据,获得的结果采用Ciphergen公司的Biomarker wizard和Biomarker Patterns System软件进行分析。结果成熟卵泡液组与窦卵泡液组蛋白质谱相比,有4种蛋白质在含量上有显著差异,2种蛋白质在成熟卵泡液组表达上调,2种蛋白质表达下降。结论成熟卵泡液与窦卵泡液蛋白质谱存在显著差异,差异蛋白可能与卵泡发育、卵子受精密切相关。Objective To study the differentially expressed proteins between mature and antral follicles and identify the proteins crucial for follicle development and oocyte fertilization. Methods Mature follicular fluids were obtained from 48 women after oocyte collection during in vitro fertilization （1VF）, and antral follicular fluids were collected from 21 women by follicular punture. The proteins in the follicular fluids were detected by surface-enhanced laser desorption/ionization-time of flight-mass spectrometry （SELDI-TOF-MS） and weak cation-exchange proteinchip （WCX-2）. The data were read with PBS II -C type proteinchip reader and analyzed with Biomarker Wizard and Biomarker Patterns SoRware of Ciphergen Company. Results In comparison with those in the antral follicular fluid, the concentration of 4 proteins were significantly diffent in mature follicular fluid, including two up-regnlated and two down-regnlated proteins. Conclusion Significant variation occurs in the proteomics of mature follicular fluid, and the differentially expressed proteins may have close relation to follicle development and oocyte fertilization.

关 键 词：表面增强激光解吸附离子化时间飞行质谱 蛋白质芯片 人卵泡液 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]