西伯利亚鲟不同性别与卵巢发育时期血液性类固醇激素的差异与判别分析  

The difference of plasma sex steroid levels and discriminant analysis of males and females in different ovarian developmental stages in cultured Acipenser baerii baerii

作　　者：张涛[1] 章龙珍[1] 赵峰[1] 庄平[1] 王斌 冯广朋[1] 黄晓荣[1] 

机构地区：[1]中国水产科学研究院东海水产研究所农业部海洋与河口渔业重点开放实验室,上海200090 [2]浙江省杭州千岛湖鲟龙科技开发有限公司,杭州311700

出　　处：《海洋渔业》2008年第1期19-25,共7页Marine Fisheries

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(2007M01);国家高技术研究发展计划(863计划)项目(2004AA603110);国家“十五”重点科技攻关计划项目(2004BA526B0114);上海高校水产养殖学E-研究院建设项目(E03009)

摘　　要：利用电化学发光免疫分析法(ECLIA)测定了人工养殖西伯利亚鲟(Acipenser baerii baerii)不同性别和卵巢发育时期血浆中睾酮(T)和雌二醇(E2)的含量。利用活组织取样的方法,根据性别和卵子颜色及大小将实验鱼分为成熟雄鱼(M)、雌鱼Ⅲ期、Ⅳ期和Ⅴ期共4个组。研究结果表明,西伯利亚鲟血浆T含量雄鱼高于雌鱼(P<0.01),且雄鱼与雌鱼Ⅲ期和Ⅴ期组有极显著性差异(P<0.01),而E2含量除与雌鱼Ⅳ期有极显著性差异外(P<0.01),与雌鱼Ⅲ期和Ⅴ期组无显著性差异(P>0.05),雄鱼表现为T含量和T/E2比值高,而E2含量低。雌鱼随卵巢的发育T和E2含量均表现为"先上升,后下降"的趋势,即Ⅳ期组性类固醇激素的含量明显高于Ⅲ期和Ⅴ期,且差异极显著(P<0.01)。根据血浆中T和E2的含量建立了西伯利亚鲟性别和性腺发育时期的4个判别函数:YM=-11.322+0.043T+0.153E2,YⅢ=-1.390+0.000T+0.061E2,YⅣ=-18.074+0.032T+3.305E2,YⅤ=-2.316+0.785T+0.008E2,经检验总体判别准确率为95%,其中对性别的判别准确率为100%,对雌鱼卵巢发育时期的判别准确率为93%。Plasma testosterone （T） and 17β-estradiol （E2 ） concentrations of different sex and ovarian developmental stages of cultured Siberian sturgeon （Acipenser baerii Brandt ） were measured by Electro- Chemiluminescence Immunoassay（ECLIA）. Biopsy method was used to obtain generative tissue. According to sex, color and diameter of eggs, sturgeons were divided into four groups ： Male （ M）, Female （ stage Ⅲ, Ⅳ and Ⅴ ）. Five individuals for each groups were sampled, and all fish in each sampling were anesthetized with clove oil（0. 1 ml/L）. Blood samples were collected from the caudal vein, and 5 ml blood samples were injected into glass tubes with anticoagulant of heparinize lithium. After centrifugation, the plasma was stored at - 20 ℃ until processing. The results showed that T concentrations of male were significantly higher than those of female （P 〈 0.01 ）, while E2 concentrations were no significantly different between male and female in different ovarian development stages （ P 〉 0.05 ） except stage Ⅳ. Male Siberian sturgeon had higher T concentrations and ratio of T/E2, but lower E2 concentrations. With the development of ovarian, plasma concentrations of T and E2 increased significantly during vitellogenesis, and reached the highest at the tertiary yolk stage （stage Ⅳ ）. When vitellogenesis was completed, T and E2 levels declined significantly at the migratory nucleus stage（stage Ⅴ ） before ovulation, plasma concentrations of T and E2 of female at stage Ⅳ were significantly higher than at stage Ⅲ and stage Ⅴ （P 〈0.01 ）. The ratio of T/E2 increased from stage Ⅲ to stage Ⅴ, but there were no significantly different between the three ovarian stages （P 〉 0.05 ）. According to the plasma cincentrations of T and E2 , four discriminant functions of sex and gonal development stages of Siberian sturgeon were then produced ： YM = - 11. 322 ＋ 0. 043T ＋ 0. 153E2, YⅢ = - 1. 390 ＋ 0. 000T ＋ 0. 061 E2, YⅣ= - 18. 074 ＋ 0. 032T ＋

关 键 词：西伯利亚鲟 性别判别 卵巢发育 睾酮 雌二醇 

分 类 号：S917[农业科学—水产科学]