喜树叶片次生代谢产物的高效液相图谱分析  

Determination of Secondary Metabolites in Leaves of Camptotheca acuminata by HPLC

作　　者：王博文[1] 王萍[1] 潘华[1] 王洋[1] 阎秀峰[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《东北林业大学学报》2008年第3期49-50,共2页Journal of Northeast Forestry University

基　　金：黑龙江省杰出青年基金资助项目(JC-02-11)

摘　　要：借鉴中药指纹图谱的思想，建立了一种快速、全面分析喜树（Camptotheca acuminata）叶片次生代谢产物的高效液相图谱分析方法：C18色谱柱（250mm×4．6mm，5μm），以甲醇-2％（体积分数）乙彰水（mL/mL）为流动相进行梯度洗脱（85min），检测波长210～400nm，样品用甲醇超声提取。运用该方法从喜树叶片中检测出11种次生代谢产物。A rapid chromatography method for the determination of secondary metabolites in leaves of Camptotheca acuminata by HPLC was established by borrowing the idea from Chinese medicine fingerprint. The secondary metabolites were separated with gradient elution performed on a C18 column （250 mm×4.6 mm, 5 μm） within 85 minutes using methanol aqueous solution （2 mL acetic acid in 100 mL H2O） as the mobile phase, and detected at 210-400 nm by a diode-array detector. Secondary metabolites were ultrasonically extracted by methanol. Eleven compounds were isolated from C. acuminata leaves.

关 键 词：喜树叶片 高效液相图谱 次生代谢产物 

分 类 号：Q946.8[生物学—植物学] S792.99[农业科学—林木遗传育种]