加味小柴胡颗粒及其组方对ITP模型小鼠免疫功能的影响  

The impact of the Jiawei Xiaochaihu particle and it's prescription on immune function of ITP mice

作　　者：王缨[1] 夏卫军[2] 

机构地区：[1]南京中医药大学附属常州市中医医院,常州213003 [2]南京中医药大学,南京210029

出　　处：《中国免疫学杂志》2008年第2期131-134,共4页Chinese Journal of Immunology

基　　金：2005年江苏省中医药管理局基金资助项目(项目号H05001)

摘　　要：目的:观察加味小柴胡颗粒及其组方对ITP模型小鼠免疫器官和免疫功能的影响。方法:建立ITP模型小鼠,将之分为5组:(1)正常组;(2)模型组;(3)加味小柴胡颗粒组;(4)小柴胡颗粒组;(5)丹参三七颗粒组。于造模后第7天开始分组给药,给药体积为30ml/kg体重,每日给药一次,共14天。造模21天眼球取血,流式细胞仪测定外周血T淋巴细胞亚群,双抗体夹心酶联免疫法(ELISA)测外周血血小板抗体,放射免疫法(RIA)测定外周血淋巴细胞因子IL-2、IL-10;处死后,取出胸腺、脾脏称重,计算免疫器官指数。结果:ITP模型小鼠免疫器官脾脏肿大,指数上升;外周血血小板抗体水平增高;T淋巴细胞亚群失调,CD8升高,CD3、CD4下降,CD4/CD8下降;Th1类细胞因子IL-2亢进,Th2类细胞因子IL-10受抑;与正常组比较有显著差异(P<0.05)。加味小柴胡颗粒组与小柴胡颗粒组脾脏指数下降,血小板抗体水平下降,CD3、CD4、CD4/CD8上升,CD8下降,下调IL-2,上调IL-10,与模型组相比有显著差异(P<0.05),而丹参三七颗粒组无差异(P>0.05)。结论:ITP模型小鼠免疫器官及功能异常,加味小柴胡颗粒及小柴胡颗粒有调节作用,丹参三七颗粒则无影响。Objective：To observe the impact of the Jiawei Xiaochaihu particle and it＇s prescription on immune organ and immune function of ITP mice.Methods：The ITP mice were divided into five groups at random：（1）Nomal group;（2）ITP model group;（3）Jiawei Xiaochaihu particle group;（4）Xiaochaihu particle group;（5）Danshen and Sanqi particle group.All ITP mice were administrated 7days later by 30ml per kilogram the rat＇s weight once a day for 14 days.21 days after the model mice were made,peripheral blood T lymphocyte subpopulation was detected with flow cytometry.Antibody against platelets was measured by ELISA.Peripheral blood IL-2 and IL-10 were measured by radio immuno assay（RIA）.Thoracic gland and spleen were weighted to compute the immune organ index.Results：Spleens of ITP mice had intumesce of spleens and a rise of the index;Peripheral blood platelet antibody was also increased;A disproportion was found in T lymphocyte subpopulation,CD8 upgrade,CD3 and CD4 descent,CD4/CD8 also descent.As one of the Th1 cytokine,IL-2 expression accentuated while IL-10,the kind of Th2 cytokine,was inhibited.There were significances when compared with the nomal group（P〈0.05）.We could find that spleen index and PLT antibody level descent,CD3,CD4 and CD4/CD8 increased,with down regulation of IL-2 and up regulation of IL-10 in the mice of Jiawei Xiaochaihu particle group and Xiaochaihu particle group.A siginificance was found when these groups were compared with the model group（P〈0.05）,but nothing was found between the Danshen and Sanqi particle groups and model group（P〉0.05）.Conclusion：There are indeed dysfunction in immune organ and its function of ITP mice.Jiawei Xiaochaihu particle and Xiaochaihu particle can exert a regulation,but the Danshen and Sanqi particle not.

关 键 词：小柴胡颗粒 丹参 三七 ITP 动物模型 免疫 

分 类 号：R3[医药卫生—基础医学]