四川草科鸡IL-15 cDNA的克隆与序列分析  

cDNA Cloning and Sequence Analysis of Interleukin-15 Gene in Sichuan Caoke Chicken

作　　者：刘蕊娜[1] 邹年莉[1] 王红宁[1] 柳萍[1] 黄勇[1] 

机构地区：[1]四川农业大学动科院动物预防医学生物工程实验室,四川雅安625014

出　　处：《中国家禽》2008年第4期8-11,共4页China Poultry

基　　金：四川省青年科学基金(2007Q14-034)

摘　　要：根据GenBank上登录的鸡白介素15(IL-15)基因序列,设计合成一对特异性引物,以ConA诱导的鸡脾淋巴细胞提取的总RNA为模板进行RT-PCR扩增,并克隆到pMD18-T载体测序。测序结果表明,四川草科鸡IL-15开放阅读框(ORF)由564个核苷酸组成,编码187个氨基酸,与GenBank中公布的鸡白介素15基因进行比较,核苷酸和氨基酸同源性为98.6%～99.5%。生物信息学分析表明,该基因编码的蛋白具有亲水性,有很强的抗原性,共有3个潜在的N-糖基化位点,可能存在3个蛋白激酶C磷酸化位点,存在2个酪蛋白激酶Ⅱ磷酸化位点。The chicken intedeukin-15（IL-15） was amplified from ConA-activated chicken splenocytes by RT-PCR using a pair of primers which were designed and synthesized according to the published ChIL-15 gene sequence in GenBank. The gene was inserted into pMD18-T vector and sequenced. The result of sequence analysis showed that the open reading frame （ORF） of Sichuan Caoke chicken IL-15 gene was 564 bp,encoding 187 amino acids, The homology of Caoke chicken ChIL-15 gene with others in GenBank were 98.6% to 99_5% in nucleotide and amino acids level. Bioinformatics analysis of this sequence showed that the protein of ChIL-15 was hydrophilic and highly antigenic,includes three potential N-glycosylation site,three potential protein kinase C phosphorylation site,two potential casein kinase Ⅱ phosphorylation site.

关 键 词：四川草科鸡 白细胞介素-15 基因克隆 序列分析 

分 类 号：Q78[生物学—分子生物学] Q343.1