辽宁省禽大肠杆菌血清型分布与大肠杆菌病防治措施研究  

Serotype Distribution of Avian Colibacillus in Liaoning Province and the Prevention Measures

作　　者：李玉文[1] 李惠兰[1] 张鹏[1] 李凤元[1] 李作义[2] 杨本勇[1] 李胜杰[1] 闫明媚[1] 吴洪涛[1] 张雷[1] 

机构地区：[1]辽宁省动物疫病预防控制中心,辽宁沈阳110161 [2]辽宁省动物卫生监督管理局,辽宁沈阳110003

出　　处：《中国家禽》2008年第4期22-24,共3页China Poultry

摘　　要：以病原菌分离、血清型鉴定及本动物致病性试验对流行于辽宁省致病性禽大肠埃希氏菌的血清型分布、致病性以及用蜂胶苗防治该病进行了研究。2001至2005年从14市的病死禽和死胚中分离出大肠杆菌226株,定型105株,其中O78型占29.52%(31/105);O109型占10.48%(11/105)。结果证明O78、O109血清型是流行于本省致病性禽大肠埃希氏菌的绝对优势血清型。致病性试验研究证明,O109型菌对鸡的致病性强于O1、O2、O78居第一位。用2001年分离的优势菌株的11个血清型大肠杆菌蜂胶苗和大肠杆菌新城疫二联蜂胶苗对鸡免疫,从2005年分离的优势菌株中选O78、O109、O1、O142、O93型菌分别以致死量进行攻毒,保护率均达100%。免疫鸡与发病鸡同居感染试验,100%健康存活。Isolation of pathogens and identification of serotype and the challenge experiment had been expatiated in this study. 226 strains colibucillus had been isolated from dead avian and embryos in 14 cities from 2001 to 2005.The serotypes of 105 strains colibucillus had been identified,of which the serotype of 078 taken the percentage of 29.52%（31/105）and 0109 was 10.48%（11/105）.The results showed that O78 and O109 were the predominant serotypes in Liaoning province. The challenge experiment showed that O109 was the most pathogenic serotype. The chickens were inoculated with colibacillosis proplis emulsion vaccine and ND-colibacillosis bivalent conjucant proplis emulsion vaccine made of 11 serotypes avian colibucillus isolated in 2001 ,then challenged with O78,O109,O1,O142 and O93,and the protect rate were all 100%. The livability of vaccined chicken was 100% when they lived with infected ones.

关 键 词：大肠杆菌 血清型 辽宁 大肠杆菌病 防治措施 

分 类 号：S858.3[农业科学—临床兽医学] S852.612[农业科学—兽医学]