麻黄药材HPLC指纹图谱的研究  

Fingerprints of Herba Ephedrae by HPLC

作　　者：符继红[1] 张丽静[1] 

机构地区：[1]新疆大学理化测试中心,新疆乌鲁木齐839646

出　　处：《中成药》2008年第2期163-166,共4页Chinese Traditional Patent Medicine

基　　金：新疆大学青年教师科研启动基金项目(QN040125)

摘　　要：目的:采用高效液相色谱法建立麻黄药材的指纹图谱,为其品质控制提供依据。方法:采用RP-HPLC,CLC phenyl(5μm4.6 mm×150 mm)色谱柱,乙腈-0.1%磷酸水溶液梯度洗脱,流速1.0 mL/min,检测波长215 nm。结果:10批产各地麻黄药材指纹图谱有17个共有峰,峰面积之和大于总峰面积的90%。对各产地的药材进行了相似度比较,建立了麻黄药材的HPLC指纹图谱。结论:采用该法可为控制麻黄药材的内在质量提供依据。AIM：To study the HPLC fingerprints and to establish a sensitive and specific method for controlling the quality of Herba Ephedrae. METHODS ： HPLC method was set up ,using CLCphenyl column（5 μm 4, 6 mm × 150 mm） with gradient elution, the mobile phase was comprised of acetonitrile and phosphoric acid（0.1% ）, the UV detection wavelength was at 215 nm, with a flow rate of 1, 0 mL/min. RESULTS： There were 17 common peaks in the fingerprints of Herba Ephedrae based on 10 habitats. Sum of peak areas was larger than 90% total area. The similarity was compared and the HPLC fingerprints was set up. CONCLUSION： The quality of Herba Ephedrae can be controlled effectively by HPLC fingerprints.

关 键 词：麻黄 高效液相色谱法 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]