正交法优化缬草挥发油超临界CO_2流体提取工艺  

Supercritical CO_2 extraction of essential oil from Valeriana officinalis by orthogonal design

作　　者：张建超[1] 方颖[1] 刘焱文[1] 王磊[1] 邹兴一[1] 

机构地区：[1]湖北中医学院药学院,湖北武汉430061

出　　处：《中成药》2008年第2期201-203,共3页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(30500053)

摘　　要：目的:采用正交设计法,对缬草挥发油超临界CO2流体提取工艺参数进行优化。方法:采用L9(34)正交实验萃取压力(A),萃取温度(B),萃取时间(C)和分离温度(D)作为四个因素,每个因素选3个水平,以挥发油样品中乙酸龙脑酯的含量来确定最优化提取条件。结果:A和D对提取的乙酸龙脑酯含量影响最大,C条件影响最小。结论:最优萃取条件是:萃取压力:12 MPa,萃取温度:45℃,萃取时间:1 h,分离温度:35℃。在此条件下萃取缬草挥发油得率最高,样品中乙酸龙脑酯含量也最高。AIM： To study the parameters of supercritical CO2 extraction of essential oil from valeriana efficinalis by orthogonal design. METHODS ： Four factors, such as extraction pressure, extraction temperature, extraction time and separation temperature were chosen by the observation of orthogonal design, each factor was assigned to three levels. Bornyl acetate content was selected as a marker in a position to determine optimal extraction. RE- SULTS ： Pressure and temperature were the main factors in effecting the extraction of bornyl acetate, extraction time was minor factor relatively. CONCLUSION： The optimal sure ： 12 MPa, extraction temperature ：45 ℃, extraction recoveries of essential oils and bornylacetate. extraction is as follow, parameters were extraction pres- time ： 1 hour, separation temperature ： 35 ℃, it gave the best

关 键 词：正交设计 缬草 挥发油 超临界CO2萃取 

分 类 号：R944.27[医药卫生—药剂学]