HPLC同时测定维C银翘片中绿原酸、对乙酰氨基酚、维生素C、马来酸氯苯那敏的含量  

Determination of chlorogenic acid、paracetmol、Vitamin C and chlorphenamine maleate in Vitamin C Yinqiao Tablets by HPLC

作　　者：高光伟[1] 冯向东[2] 黄海欣[2] 

机构地区：[1]南阳市中心医院,河南南阳473000 [2]河南省南阳市药品检验所,河南南阳473000

出　　处：《中成药》2008年第2期207-210,共4页Chinese Traditional Patent Medicine

摘　　要：目的：建立维C银翘片中绿原酸、对乙酰氨基酚、维生素C、马来酸氯苯那敏的含量测定方法。方法：采用HPLC法,使用C18柱,流动相：甲醇-乙腈-0.02%磷酸（5∶10∶85）;流速：1.0 mL/min;柱温：35℃;检测波长：310 nm和219 nm。结果：绿原酸、对乙酰氨基酚、维生素C、马来酸氯苯那敏进样量分别在0.03～0.13μg、0.94～4.68μg、0.49～2.44μg、0.01～0.05μg范围内呈良好线性关系,平均回收率（n=5）均不低于98%。结论：本法简便、快捷,结果令人满意,可作为维C银翘片的质量控制方法之一。AIM： To establish a method of determining the content of chlorogenic acid, paracetmol, vitamin C and chlorphenamine maleate in Vitamin C Yinqiao Tablets. METHODS： The separation was performed in the C18 colunm with the mobile phase of methanol-acetonitril-0.02% phosphate acid （ 5 ： 10 ： 85 ）. The flow rate was 1.0 mL/min with the wavelength at 219 nm and 310 nm, the temperature of column was 35 ℃. RESULTS： The calibration curves were linear in the ranges of 0.03 -0.13 μg for chlorogenic acid,0.94-4.68 μg for paracetmol, 0.49-2.44 μg for Vitamin C ,0.01 -0.05 μg for chlorphenamine maleate, the average recoveries were not less than 98% ,respectively. CONCLUSION： The method is simple, rapid and with satisfactory results. It is suitable for quality control of Vitamin C Yinqiao Tablets.

关 键 词：HPLC 维C银翘片 绿原酸 对乙酰氨基酚 维生素C 马来酸氯苯那敏 

分 类 号：R927.2[医药卫生—药学]