HPLC同时测定茵山莲颗粒中野黄芩苷和栀子苷含量  

Determination of geniposide and scutellarin in Yinshanlian Granules by HPLC

作　　者：刘兴超[1] 宋金玉[2] 徐海燕[1] 朱鸿敏[1] 门金玉[1] 赵怀清[1] 

机构地区：[1]沈阳药科大学,辽宁沈阳110016 [2]山西生物应用职业技术学院中药系,山西太原030031

出　　处：《中成药》2008年第2期210-213,共4页Chinese Traditional Patent Medicine

摘　　要：目的:建立同时测定茵山莲颗粒(无糖型)(茵陈,半枝莲、栀子等)中栀子苷和野黄芩苷含量的高效液相色谱法。方法:采用Diamonsil C18(200 mm×4.6 mm,5μm)柱分离,以乙腈(A)和0.1%磷酸水溶液(B)进行梯度洗脱,程序为:5%A^33%A(0~30 min):33%A^5%A(30~31 min);5%A(31~35 min),在240 nm处检测。结果:野黄芩苷在0.032~0.288 mg/mL范围内,栀子苷在0.009 6~0.086 4 mg/mL范围内质量浓度与峰面积均呈良好的线性关系;野黄芩苷和栀子苷的平均回收率分别为100.6%(RSD=0.80%,n=9),102.6%(RSD=1.1%,n=9)。结论:本法操作简便,准确度高,重现性好,可作为茵山莲颗粒(无糖型)质量控制方法之一。AIM： To develop a HPLC method for determining scutellarin and geniposide in Yinshanlian Granules（Herba Artemisiae scopariae ,Herba Scutellariae barbatae , Fructus Gardemiae , etc ）. METHODS： The analysis was performed on Diamonsil C18 （200 mm ×4.6 mm,5 μm） with mixture of acetonitrile （A） and 0.1% phosphoric acid solution as mobile phase in gradient mode. The concentration of solvent A were 5%, 33%, 5% and 5% at 0, 30, 31 and 35 min, respectively. The detection wavelength was set at 240 nm and the column temperature was at 30 ℃. RESULTS：The linear calibration curves were obtained in the concentration range of 0. 032 -0. 288 mg/mL for scutellarin and 0. 009 6 - 0. 086 4 mg/mL for geniposide. The average recoveries of scutellarin and geniposide were 100.6% （RSD =0. 80%, n =9）, 102.6% （RSD = 1.1%, n =9） ,respectively. CONCLUSION： The method is quick, simple,and reproducible, which can be used to control the quality of Yinshanlian Granules.

关 键 词：茵山莲颗粒(无糖型) 野黄芩苷 栀子苷 HPLC 

分 类 号：R927.2[医药卫生—药学]