全氟化碳腹腔注射对内毒素致急性肺损伤大鼠预防作用的实验研究  

The preventive effects of intraperitoneal injection of perfluorocarbon on acute lung injury in rat

作　　者：樊毫军[1] 刘书盈[2] 张健鹏[1] 赵晓巍[1] 高红梅[1] 刘又宁[3] 

机构地区：[1]武警总医院呼吸科,北京100039 [2]济南军区总医院呼吸科,济南250031 [3]解放军总医院呼吸科,北京100853

出　　处：《中国危重病急救医学》2008年第2期100-103,132,共5页Chinese Critical Care Medicine

基　　金：国家“十五”重大攻关课题基金资助项目（2003BA712A07-01）;武警部队科研课题资助项目（WZ2006016）

摘　　要：目的观察腹腔注射全氟化碳（PFC）对内毒素致急性肺损伤（ALI）的预防作用。方法63只雄性健康Wistar大鼠随机分为空白对照组（NC组，9只）、内毒素脂多糖（LPS）致伤组（LPS组，27只）和8碳PFC（C8F18）预防组（预防组，27只）。NC组腹腔注射质量分数为2％的戊巴比妥（40mg／kg）麻醉后2h处死,LPS组与预防组分别于实验前48h腹腔注射生理盐水（15ml／kg）或C8F18（15ml／kg），实验当日腹腔注射2％戊巴比妥后静脉注射LPS7mg／kg，于2、4和6h分批处死大鼠。比较3组各时间点动脉血氧分压（PaO2）、肺组织病理损伤评分、肺系数（右肺湿重／体重）、血液和支气管肺泡灌洗液（BALF）中肿瘤坏死因子-α（TNF-α）浓度。结果LPS组和预防组PaO2均明显低于NC组（P均〈0．05）；LPS组致伤后2、4和6hPaO2与预防组同期比较差异均无统计学意义（P均〉0．05）。LPS组肺组织病理学改变主要为肺泡间隔增宽，大量白细胞渗出、聚集，部分肺泡萎陷不张，腔中可见渗出液、出血；与LPS组比较，预防组肺组织病理变化无明显改善；病理损伤评分也无明显降低。LPS组与预防组血液和BALF中TNF-α的浓度均显著高于NC组（P均〈0．01）；与LPS组比较，预防组血清TNF-α浓度在2、4和6h无明显改变，BALF中TNF-α浓度在6h时明显低于LPS组（P〈0．05）。结论腹腔注射C8F18原液15ml／kg不能有效改善LPS所致ALI的PaO2下降及肺组织病理损伤，但对肺内TNF-α释放具有一定的抑制作用。Objective To evaluate the preventive effects of intraperitoneal injection of perfluorocarbon （PFC） on acute lung injury （ALI） in rat. Methods Sixty-three male Wistar rats were randomly divided into normal control （NC） group, lipopolysaccharide （LPS） group and C8F18 group. Rats in NC group were sacrificed 2 hours after anesthesia, and in LPS group and C8F18 group rats were either treated with normal saline or C8F18 15 ml/kg intraperitoneally 48 hours before LPS challenge. ALI was reproduced by intravenous injection of 7 ml/kg LPS, and the extent of lung injury was assessed by arterial partial pressure of oxygen （PaO2） level, histological examination, right lung wet weight/body weight （W/D） ratio, tumor necrosis factor-α （TNF-α） level in serum and broncho alveolar lavage fluid （BALF） at 2, 4, 6 hours after injury. Results PaO2 in LPS group and C8F18 group was significant lower than that in NC group （both P〈0. 05）. There were no difference in PaO2 between LPS group and C8F18 group at 2, 4, 6 hours after injury （all P〉 0. 05）. Compared with NC group, TNF-α level in blood and BALF increased obviously in LPS group and C8F18 group （both P〈0. 05）. There was no significant change in content of TNF-α in C8F18 group BALF at 6 hours was significantly lower than that in LPS group （P〈0.05）. Conclusion Intraperitoneal administration of C8F18 （15 ml/kg） can not attenuate pathological changes or improve PaO2 in rats with ALI induced by LPS in a short time.

关 键 词：全氟化碳 内毒素 肺损伤 急性 

分 类 号：R563.8[医药卫生—呼吸系统]