氨基胍对失血性休克兔血流动力学和一氧化氮的影响  

Effect of aminoguanidine.on hemodynamics and nitrogen monoxide in hemorrhagic shock in rabbit

作　　者：高治平[1] 廖端芳[1] 李波平[1] 刘德平[2] 刘建芝[2] 唐朝克[3] 

机构地区：[1]南华大学药物药理研究所,湖南衡阳421001 [2]南华大学机能实验中心,湖南衡阳421001 [3]南华大学心血管病研究所,湖南衡阳421001

出　　处：《中国危重病急救医学》2008年第3期176-179,共4页Chinese Critical Care Medicine

基　　金：基金项目：湖南省教育厅基金资助项目（04C539）

摘　　要：目的观察氨基胍（AG）在失血性休克复苏中的作用机制。方法采用股动脉放血法复制家兔失血性休克模型，将20只家兔随机均分为生理盐水复苏对照组和AG复苏组；用Pclab生物信号采集系统分别记录休克前、休克30min、休克150min、复苏30min、复苏180min的血流动力学参数，并用硝酸还原酶法测定相应时间点的血清一氧化氮（NO）水平。结果两组复苏前动脉收缩压（SAP）、心率（HR）、平均动脉压（MAP）、左心室收缩压（LVSP）、心室内压最大上升和下降速率（4-dp／dt max）等血流动力学参数与血清NO含量比较差异均无统计学意义。与休克前比较，两组休克30min和150min血流动力学参数显著下降，NO含量显著升高（P〈0.05或P〈0．01）。复苏后，两组均能改善失血性休克家兔血流动力学参数，同时AG复苏能显著降低失血性休克家兔血清NO含量，而生理盐水复苏不能阻断血清NO升高；两组比较，AG具有更持久、更有效的抗失血性休克作用（P〈0.05或P〈0．01）。结论氨基胍能改善失血性休克家兔血流动力学参数，降低血清NO水平，从而起到抗失血性休克作用。Objective To explore the effect of aminoguanidine （AG） on hemodynamics and nitric oxide （NO） in hemorrhagic shock in rabbit. Methods Shock was produced in twenty rabbits by femoral artery blood letting, and they were randomly divided into two groups with treatment with saline （control group） or AG （each n = 10）. Pclab system and nitric acid reductase methods were performed to detect hemodynamics parameters and NO levels at preshock, postshock （30 minutes and 150 minutes） and 30 minutes and 180 minutes after resuscitation respectively. Results No significant differences were observed in hemodynamics parameters and NO levels between the two groups before resuscitation. Whereas after resuscitation, hemodynamics parameters such as systolic arterial pressure （SAP）, heart rate （HR）, mean arterial pressure （MAP）, left ventricular systolic pressure （LVSP） and the max and minimum first derivative of left ventricular pressure （±dp/dt max） were all improved greatly in the two groups. AG decreased serum NO level significantly, while saline did not have such effect, suggesting that AG had a longer and more efficient effect against hemorrhagic shock （P〈0.05 or P〈0.01）. Conclusion AG can improve hemodynamics parameters and decrease NO level in hemorrhagic shock in rabbit.

关 键 词：失血性休克 氨基胍 血流动力学 诱生型一氧化氮合酶 一氧化氮 

分 类 号：R459.7[医药卫生—急诊医学]