烯醇还原酶不对称生物还原激活的C=C键的研究最新进展  

Recent advances in asymmetric bioreduction of activated C=C bonds using enoate reductases

作　　者：杨隽娴[1] 吴小飞[1] 高强[1] 刘均洪[1] 

机构地区：[1]青岛科技大学化工学院,山东青岛266042

出　　处：《化学工业与工程技术》2008年第1期20-25,共6页Journal of Chemical Industry & Engineering

基　　金：国家自然科学基金资助课题;批准号:20176019

摘　　要：在消耗NAD(P)H的条件下,烯醇还原酶不对称还原带有吸电子基团的烯烃的C=C键,生成相应的非外消旋烷烃,同时产生2个手性碳原子中心。为了避免需要外部的辅因子再循环,迄今为止,主要还是用整个微生物细胞进行生物转化,但细胞内由于有其他酶的竞争,使得这种生物转化通常表现出较低的立体选择性和较多的副反应。利用在同一宿主中共表达烯醇还原酶及相应催化NAD(P) H循环再生的氧化还原酶,有望解决以上的缺点,使得生物转化表现出较高的立体选择性,并可在制备规模上专一性生物还原激活的C=C键。At the expense of NAD（P）H, the asymmetric bioreduction of alkenes bearing an electronwithdrawing group using enoate reductases yields the corresponding non-racemic alkanes, it creates two chiral carbon centers at the same time. To avoid external cofactor recycling, this biotransformation is hitherto performed using whole microbial cells, which frequently shows insufficient stereoselectivities and many side reactions because of the action of competing enzymatic activities. Co-expression of enoate reductases with the corresponding redox enzymes for NAD（P）H recycling in a suitable host enables to overcome these drawbacks to furnish highly stereoselective and bioreduce activated C=C bonds on a preparative scale.

关 键 词：烯醇还原酶 不对称 生物还原 激活C=C键 

分 类 号：Q55[生物学—生物化学]