山羊mtDNA多态性及其遗传结构的研究  

Research on the Genetic Diversity and Structure of mtDNA of 6 Indigenous Goat Breeds in China

作　　者：季香[1] 马月辉[1] 叶绍辉[2] 郭军[1] 

机构地区：[1]中国农业科学院畜牧研究所,北京100094 [2]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《云南农业大学学报》2008年第2期220-224,共5页Journal of Yunnan Agricultural University

基　　金：国家自然科学基金项目(30371026);国家自然科技资源平台项目(2005DKA21101)

摘　　要：对中国6个山羊品种(辽宁绒山羊,内蒙古绒山羊,西藏山羊,中卫山羊,济宁青山羊和龙陵山羊)的52个样本进行m tDNA的D-Loop区的全序列测定,用来研究中国山羊品种的线粒体DNA遗传多态性及系统发育。用NJ法构建单倍型系统发育树,结果发现6个山羊品种的线粒体DNA控制区聚类后得到了4个很明显的分支,即单倍型A,B,C,D.品种间遗传结构分析发现内蒙古绒山羊、辽宁绒山羊两个绒山羊品种与西藏山羊、中卫山羊3个可产绒的山羊品种聚在一起,接着与产羔皮的济宁青山羊聚在一起,最后与产肉性能强的龙陵山羊聚在一起。In this paper, 52 individuals were chosen from 6 indigenous goat breeds raised in China. Fifty-two individuals＇ complete sequences of D-Loop, the control region of mitochondrial DNA （mtD- NA）, were sequenced in order to study mtDNA genetic diversity and phylogenetic evolution of these 6 breeds. The phylogenetic tree of D-Loop was constructed with Neighbour-Joining （ N J） method and the genetic structure among the 6 breeds was studied by cluster analysis based on the genetic distance. The results showed that all of the domestic haplotypes were divided into 4 high obvious divergent clusters （A, B, C and D） and the cluster analysis showed a closer relationship among LNC, NMC, TiG and ZW goats and a farther relationship between LL goat and other goat.

关 键 词：山羊 MTDNA 遗传多样性 系统发育 

分 类 号：S827.2[农业科学—畜牧学]