肺癌患者CD4^+ CD25^+调节性T细胞的检测及临床意义  

Significance of Measurement of CD4^+ CD25^+ Regulatory T Cell in Peripheral Blood of Patients with Lung Cancer

作　　者：韩芳[1] 丁昌平[2] 顾军[3] 童建东[1] 张敏[2] 汪竹[1] 张正荣[1] 汪瑞[1] 曹春梅[2] 柳健[2] 

机构地区：[1]江苏省扬州市第一人民医院肿瘤科,225001 [2]江苏省扬州市第一人民医院中心实验室,225001 [3]江苏省扬州市第一人民医院胸外科,225001

出　　处：《放射免疫学杂志》2008年第1期77-79,共3页Journal of Radioimmanology

摘　　要：目的:检测肺癌患者外周血CD4+CD25+调节性T细胞的分布并探讨相关机制。方法:采用流式细胞仪分析66例肺癌患者外周血CD4+CD25+调节性T细胞占CD4+T淋巴细胞的比例。结果:66例肺癌患者外周血中CD4+CD25+调节性T细胞占CD4+T淋巴细胞的比例为(16.2±2.4)%,与对照组(6.19±1.5)%比较差异有显著性(P<0.05)。25例鳞癌、29例腺癌、12例小细胞癌患者外周血中CD4+CD25+调节性T细胞比例分别为(18.3±2.9)%、(15.6±1.8)%、(17.3±2.2)%,各组间比较差异无显著性(P>0.05);均显著高于对照组(6.19±1.5)%,P<0.05;34例Ⅲ期,14例Ⅳ期肺癌患者外周血中CD4+CD25+调节性T细胞比例为(15.3±2.6)%,(20.4±3.1%),均显著高于18例Ⅱ期患者(9.4±1.3)%,P均<0.05。结论:肺癌患者外周血中有CD4+CD25+调节性T细胞比例增高,且与分期有关。它可能与肺癌患者免疫功能受损有关,可作为评估肺癌患者预后的一项指标。Objective To detect the significance of changes of proportion of CD4^＋ CD25^＋ regulatory T cells in peripheral blood of patients with lung cancer. Methods The proportion of CD4^＋ CD25^＋ regulatory T cells in peripheral blood CD4^＋ T lymphocyte cells were evaluated by flow cytometric analysis in 66 patients with lung cancer and 32 controls. Results In patients with lung cancer the percentages of CD4^＋ CD25^＋ regulatory T cells were significantly higher than those in controls（ 16.2 ± 2.4% vs 6.3 ± 1.6%, P 〈 0.05）. Among the patients with lung cancer, the proportion of CD4^＋ CD25^＋ regulatory T cells in patients with squamous carcinoma （ n = 25）, adenocarcinoma （ n = 29） and small cell carcinoma （ n = 12） were all significantly higher than those in controls （ P 〈 0. 05）, but there were no obvious differences among the three groups of different pathological type （ P 〉 0.05）. The proportion of CD4^＋ CD25^＋ regulatory T cells in the peripheral blood of 34/14 lung cancer patients of Ⅲ/Ⅳ stages were significantly higher than those in 18 lung cancer patients of Ⅱ stage（15.3±2.6%,20.4±3.1% vs9.4±1.3%,P〈0.05）. Conclusion The propertion of CD4^＋ CD25 ^＋ regulatory T cells in the peripheral blood of lung cancer patients was increased and correlated with different stages of the disease. This fact might be responsible for immune suppression in lung cancer and was of prognostic value.

关 键 词：肺癌 外周血 CD4^+ CD25^+ 调节性T细胞 

分 类 号：R392.11[医药卫生—免疫学] R511[医药卫生—基础医学]