不同剂量的神经节苷脂对人脂肪组织来源的基质细胞分化为神经组织细胞的影响  

Effect of different dose of GM1 on inducing human adipose tissue-derived stromal cells into neural cells

作　　者：刘斌[1] 吴孟海[1] 张晋霞[1] 

机构地区：[1]华北煤炭医学院附属医院神经内科,唐山063000

出　　处：《临床神经病学杂志》2008年第1期33-36,共4页Journal of Clinical Neurology

基　　金：河北省教育厅自然科学科研项目(Z2005101)

摘　　要：目的探讨不同剂量的神经节苷脂(GM1)对人脂肪组织来源的基质细胞(ADSCs)分化为神经组织细胞的影响。方法分离培养人ADSCs,采用神经诱导培养基(NIM)加不同剂量的GM1对各组进行诱导培养。实验分6组:对照组,只加改良Eagle培养液(α-MEM);A组,神经诱导培养基(NIM);B组,NIM加50μmol/LGM1;C组,NIM加100μmol/LGM1;D组,NIM加200μmol/LGM1;E组,NIM加400μmol/LGM1。诱导后1h、5h、1d和5d倒置相差显微镜下连续观察各组细胞生长情况和形态变化;免疫组化法鉴定各组神经巢蛋白(Nestin)、神经元特异性烯醇化酶(NSE)、微管联合蛋白-2(MAP2)及胶质纤维酸性蛋白(GFAP)的表达情况。结果(1)诱导后,ADSCs可分化为神经细胞,在一定的时间内,分化的神经细胞数逐渐增加,于诱导后5h、1d和5dE组增加更明显(均P<0.05)。(2)各组Nestin的表达随时间逐渐增强,1d时表达最高,5d时表达减弱,除1h外,其余各时间点E组表达最高(均P<0.05);NSE和MAP2的表达随时间逐渐增强,5d时表达最高,各时间点E组表达最高(均P<0.05);GFAP的表达随时间逐渐增强,5d时表达最高,各时间点E组表达最低(均P<0.05)。结论GM1可促进人ADSCs向神经细胞分化,并呈剂量依赖性。Objective To investigate the effect of different dose of GM1 on inducing human adipose tissuederived stromal cells （ADSCs） into neural cells. Methods Human ADSCs were isolated from human liposuction tissues and cultured in NIM with different dose of GM1, which was used to induce ADSCs into neural cells. The experiment was separated into six groups： control group （α-MEM）, Group A （NIM）, group B （NIM ＋ 50 μmol/L GM1）, group C （NIM ＋100 μmol/L GM1）, group D （NIM ＋200 μmol/L GM1） and group E （NIM ＋400 μmol/ L GM1 ）. Cells＇ growth and morphologic changes were observed under invert phase-contrast microscopy. The expressions of Nestin, NSE, MAP2 and GFAP were identified by immunocytochemistry methods. Results （1） ADSCs could differentiate into neural cells in NIM with different dose of GM1. The number of neural cells differentiated from ADSCs increased in a certain period, especially in group E at each time point （all P 〈 0. 05 ）. （2） The expression of Nestin increased with the time going, reached the peak at 1 d and decreased at 5 d. Compared with the other groups, the expression in group E was the highest except 1 h time point （ all P 〈 0. 05 ）. The expressions of NSE, MAP2 and GFAP increased with the time going, and reached the peak at 5 d. Compared with the other groups, the highest expressions of NSE, MAP2 and lowest expression of GFAP were found in group E at the same time （all P 〈 0. 05 ）. Conclusion GM1 can induce human ADSCs into neural cells, and its differentiation depends on dose of GM1.

关 键 词：脂肪组织 基质细胞 神经细胞 神经节苷脂 胶质纤维酸性蛋白 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]