甲胺对兔心血管内皮的慢性毒性(英文)  

Chronic toxicity of methylamine on cardiovascular endothelium of rabbits

作　　者：罗文英[1] 林哲绚[1] 李慧[1] 罗文鸿[1] 

机构地区：[1]汕头大学医学院中心实验室,广东汕头515031

出　　处：《中国药理学与毒理学杂志》2008年第1期24-30,共7页Chinese Journal of Pharmacology and Toxicology

基　　金：国家自然科学基金项目(30271139);广东省自然科学基金项目(021224)~~

摘　　要：目的探讨甲胺在体内慢性分布是否导致氨基脲敏感胺氧化酶(SSAO)活性上调从而诱导心血管内皮损伤。方法新西兰兔灌胃给予甲胺100mg.kg-1,每日1次,连续6个月,隔周称量体重调整给药量。分析动脉血中循环内皮细胞数、血清NO浓度和主动脉内皮细胞的超微结构。高效液相色谱法检测血浆SSAO活性及甲醛浓度。结果甲胺组兔动脉血中内皮细胞数、血清NO浓度、血浆SSAO活性及甲醛浓度明显增高。主动脉内皮细胞超微结构显示核内大量包函体、核浓缩和核分裂等病理形态学改变。结论低剂量甲胺在体内慢性分布能够诱导SSAO活性上调而引起心血管内皮损伤。AIM To study whether chronic administration of methylamine may induce elevation of semicarbazide-sensitive amine oxidase （ SSAO ） activity and initiate the injury of cardiovascular endothelium. METHODS New Zealand rabbits were treated with methylamine hydrochloride （ 100 mg·kg^-1 ） by ig, once a day for 6 months. The rabbits were weighed every other week and the dosage was adjusted according to the body weight. The number of circulating endothelial cells （CEC） in the arterial blood, nitric oxide （NO） concentration in the serum and uhrastructure of endothelial cells of aorta were assessed. The plasma SSAO activity and formaldehyde concentration were assessed by liquid chromatography. RESULTS The number of CEC, NO concentration, levels of SSAO activity and formaldehyde concentration in the methylamine group were increased significantly, compared with the control group. Uhrastructure of endothelial cells in the methylamine group showed inordinate morphological changes （ multiple intranuclear inclusions, karyopyknosis and karyorrhexis ）. CONCLUSION Chronic administration of methylamine can induce the elevation of SSAO activity diovascular endothelium. and initiate the injury of cardiovascular endothelium.

关 键 词：甲胺 氨基脲敏感胺氧化酶 甲醛 内皮 血管 毒理学 

分 类 号：R114[医药卫生—卫生毒理学]