绵羊Gastorkine-1基因的克隆与序列分析  

Molecular Cloning and Sequence Analysis of Ovine Gastorkine-1

作　　者：乔新安[1] 杨国宇[1] 王艳玲[1] 王月影[1] 韩立强[1] 朱彦彩[1] 都军霞[1] 范沛[1] 

机构地区：[1]河南农业大学动物生理生化实验室,河南郑州450002

出　　处：《华北农学报》2008年第1期38-40,共3页Acta Agriculturae Boreali-Sinica

基　　金：河南省重点科技攻关项目(0523010500)

摘　　要：基于电子延伸序列，克隆并分析了绵羊Gastorkine—1基因。提取皱胃黏膜组织总RNA，利用设计的引物进行RT-PCR，PCR产物与pMD19-T载体连接后转化E．coli JM109感受态细胞，检测阳性克隆并测序。克隆的绵羊Gastorkine—1基因长619bp，编码185个氨基酸，与人、小鼠、猪、牛的同源性分别为82．0％，48．8％，85．4％，96．9％，推测的氨基酸序列信号肽为1～20aa，BRICHOS结构域为54～150aa，结构特征与人、小鼠、猪、牛的Gastorkine-1相一致。In order to clone and analysis the ovine Gastorkine-1, the known sequence of bovine Gastorkine-1 （NM001001148） was selected to search the GenBank ovine EST database, from which two ESTs （EE780384, EE77958） were obtained. Based on the sequence splieeosome, a pair of primers in 5＇-UTR and 3＇-UTR was designed and used to amplify the total RNA extracted from mueosal tissue of ovine abomasum by RT-PCR,then the PCR products were cloned and sequenced. The results showed that ovine Gastorkine-1 sequence shared 82.0% ,48.8% ,85.4% and 96.9% simi- larity with the eorresonding genes of human, mouse, pig and cattle, respectively. The signal peptide of predicted amino acid sequence contained 1 - 20 aa and the structural domain BRICHOS had 54 - 150 aa, presenting the same structural feature to those of human, mouse, pig and cattle.

关 键 词：绵羊 胃黏膜保护因子 克隆 序列分析 

分 类 号：S811.3[农业科学—畜牧学] Q789[农业科学—畜牧兽医]