小麦新种质N95175抗白粉病基因的SSR分析  

SSR Analysis of Powdery Mildew Resistance Gene in New Wheat Germplasm N95175

作　　者：马莹莹[1] 王长有[1] 吉万全[1] 王秋英[1] 刘雪利[1] 

机构地区：[1]西北农林科技大学农学院,国家小麦改良中心杨凌分中心,陕西杨凌712100

出　　处：《华北农学报》2008年第1期199-203,共5页Acta Agriculturae Boreali-Sinica

基　　金：陕西省重大科技专项(2006KZ05-G3);国家科技支撑计划(2006BAD13B03)

摘　　要：为明确小簇麦新种质N95175抗白粉病基因的遗传效应和基因位点,采用常规分析法结合SSR技术进行抗性基因的遗传分析和分子标记研究。抗性基因常规分析结果表明,N95175分别与高感白粉病普通小麦品种陕160和陕优225两个杂交组合的F1均现高抗,F2抗感植株比例分别为115∶43和111∶48,经χ2检验,符合3∶1的显性单基因孟德尔遗传分离比例,即该抗白粉病基因为显性单基因遗传。利用208对小麦微卫星引物对N95175×陕优225的F2抗感分离群体分析结果表明,Xgwm570和Xwmc553均与抗白粉病基因连锁,遗传距离分别为13.38和12.03 cM。Xg-wm570和Xwmc553两者之间在两群体中的遗传距离为3.74 cM。利用两引物对N95175×陕160组合F2代进行标记验证分析,分析结果与接种后调查结果符合率为89.24%。根据Xgwm570和Xwmc553在小麦染色体的位置,将N95175的抗白粉病基因定位在6A染色体上。To definitude genetic effects and gene loci of powdery mildew resistance gene in new wheat-haynalidia villosa germplasm N95175, conventional analyze and SSR technology was used to analyze heredity and molecular markers of resistant gene. The results showed that F1 of N95175 × Shaanl60 and N95175 x Shaanyou225 were highly resistant to powdery mildew. The ratios of resistance plants to susceptible plants in F2 generation were 115 ： 43 and 111 ： 48, respectively. The segregation ratio fitted expected 3 ： l, so it suggested that powdery mildew resistance of N95175 was controlled by a dominant gene. By SSR analysis with 208 pairs of primers, two markers Xgwm570 and Xwmc553 generated polymorphic DNA fragments between the resistant and susceptible plants DNA pools. By analyzing the polymorphic markers in these segregating populations, Xgwm570 and Xwmc553 were linked to the resistance gene with genetic distance of 13.38 and 12.03 cM.The genetic distance between marker Xgwm570 and Xwmc553 was calculated as 3.74 cM. By using two markers, the marker certification was analyzed in N95175 × Shaanl60 F2 generation, the according rate was 89.24 % compared with inoculation. According to chromosome loci of Xgwm570 and Xwmc553, powdery mildew resistance gene in N95175 was located on wheat 6A chromosome.

关 键 词：小麦新种质 抗白粉基因 遗传效应 SSR标记 

分 类 号：S435.12[农业科学—农业昆虫与害虫防治]