水稻结实期不同粒位籽粒相关内源激素含量和关键酶活性的差异及其与品质的关系  

Difference in Hormonal Content and Activities of Key Enzymes in the Grains at Different Positions on a Rice Panicle During Grain Filling and Their Correlations with Rice Qualities

作　　者：董明辉[1] 赵步洪 吴翔宙[1] 陈涛[1] 杨建昌[1] 

机构地区：[1]扬州大学农学院,江苏省作物遗传生理国家重点实验室培育基地,江苏扬州225009 [2]扬州里下河地区农业科学研究所,江苏扬州225007

出　　处：《中国农业科学》2008年第2期370-380,共11页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(30400276,30671225);江苏省自然科学基金项目(BK2006069,BK2007071);扬州大学人才启动基金资助

摘　　要：【目的】研究灌浆期水稻穗上不同粒位籽粒内源激素含量和关键酶活性的差异及其与籽粒品质形成的关系。【方法】以中熟籼稻扬稻6号和中熟粳稻扬粳9538为材料,按照一次枝梗数将稻穗分为上、中、下三个部位,以穗中部一、二次枝梗不同粒位籽粒为研究对象。【结果】灌浆前期开花较早的籽粒中玉米素(Z)+玉米素核苷(ZR)、吲哚乙酸(IAA)、脱落酸(ABA)含量以及淀粉合成酶(SSase)、淀粉分支酶(Q酶)活性明显高于同枝梗上开花较迟的籽粒,灌浆后期则相反;籽粒开花越早,其内源激素含量以及酶活性到达最大值的时间越早,峰值也越高。灌浆前期ABA、Z+ZR、IAA含量、ABA/(Z+ZR+IAA)比值以及SSase酶和Q-酶活性与千粒重、直链淀粉累积速率、直链淀粉含量呈显著或极显著正相关,与垩白度呈显著或极显著负相关,灌浆后期则相反;灌浆前期SSase和Q-酶活性与胶稠度呈负相关,中、后期则相反;与粗蛋白含量的相关两品种存在差异,与整精米率相关不显著。【结论】灌浆期水稻穗上不同粒位籽粒内源激素含量与关键酶活性存在差异;不同粒位间籽粒米质差异的形成与籽粒内源激素含量与关键酶活性密切相关。【Objective】This study investigated the differences of hormonal content and activities of key enzymes in the grains at different positions on a rice panicle during grain filling and their correlation with rice quality. 【Method】 Two rice cultivars, Yangdao 6 （indica） and Yangjing 9538 （japonica）, were grown in field. All primary branches on a panicle were evenly divided into three parts of the upper, middle and base. The grains were numbered as 1 to 6 on a primary branch and 1 to 3 or 4 on a secondary branch from the top to base. The grains at the middle part of a panicle were measured. 【Result】 The contents of zeatin （Z） ＋ zeatin riboside （ZR）, indole -3- acetic acid （IAA） and abscisic acid （ABA） and activities of starch synthase （SSase）, starch branching enzyme （Q-enzyme） in earlier flowered grains were obviously higher than those in later flowered grains at early filling stage, and vice versa at late filling stage. The earlier the spikelets flowered, the shorter the time reaching the maximum grain-filling rate was, the higher the contents and activities were. The contents of Z＋ZR, IAA and ABA, the ratio of ABA to IAA＋Z＋ZR, and activities of SSase and Q-enzyme at early grain filling stage were positively and significantly or very significantly correlated with 1 000-grain weight, amylose accumulation rate and amylose content, but negatively and significantly or very significantly correlated with chalkiness degree, and vice versa at late grain filling stage. Activities of SSase and Q-enzyme at early grain filling stage werenegatively correlated with Gel consistency （GC）, whereas positively correlated with GC at mid and late grain-filling stages. The correlation of the hormones and enzymatic activities with crude protein content had difference in two cultivars, and the correlation with head milled rice rate was insignificant. 【Conclusion】The hormonal content and activities of key enzymes in the grains at different positions on a rice panicle during grain fi

关 键 词：水稻 粒位 激素 酶活性 品质 

分 类 号：S511[农业科学—作物学]