本溪山樱根部解磷细菌的定殖规律及其对植株生长发育的影响  

Colonization Regulation Pattern of Phosphobacteria and Its Effect on the Growth and Development of Cerasus sachalinensis

作　　者：吕德国[1] 于翠[1] 秦嗣军[1] 刘国成[1] 杜国栋[1] 

机构地区：[1]沈阳农业大学北方果树育种与生理生态研究所/沈阳农业大学园艺学院,沈阳110161

出　　处：《中国农业科学》2008年第2期508-515,共8页Scientia Agricultura Sinica

基　　金：辽宁省教育厅高等学校科学研究A类项目(051393);沈阳农业大学青年教师基金项目(2005035)

摘　　要：【目的】探讨解磷细菌定殖规律及其与本溪山樱地上和地下部生长发育的关系,为土壤管理提供参考依据。【方法】以本溪山樱为研究对象,采用土壤缩影系统并结合常规的分析方法,研究植物促生根际细菌—3株解磷细菌[Bacillus(Y4)、Pseudomonas(J6)、Flavimonas(H7)]于本溪山樱根部的定殖规律及其对根际特征和植株生长发育的影响。【结果】3种菌株定殖部位因时间变化而变化,混接处理中根内没有菌株定殖,但无论单接与混接处理3种菌株定殖密度均随时间延长而降低。接种解磷细菌后,细菌于本溪山樱根部的侵染速度较快,数量较多,放线菌和真菌的侵染能力较低,数量较少。接种Y4可较对照提高植株的净光合速率(Pn),这种效果随时间的延长逐渐减弱,而接种其它菌液处理均降低了植株的Pn。单接Y4可增加植株中全磷含量。【结论】解磷细菌的定殖数量随外来微生物侵入数量的增多而减少。外来细菌的侵入对解磷细菌的定殖影响较大。处理前期,接种假单胞杆菌(Y4)和芽孢杆菌(J6)对植株生长有一定的促进作用。【Objective】Examination of the colonization regulation of phosphobacteria and its effect on the growth and development of Cerasus sachalinensis could provide a theoretical basis for soil management.【Method】Three strains with high phosphate-dissolving ability, Bacillus （Y4）, Pseudomonas （J6） and Flavimonas （H7）, were selected using dilution plate and conventional methods from C. sachalinensis rhizosphere. The colonization regulation of phosphobacteria and its effect on rhizosphere characteristics of cherry and plant growth and development were studied. 【Result】Colonization site of three strains varied with inoculation time, and there were no strains colonized in root-inside in the mix inoculation treatment, the colonization density of bacteria decreased gradually regardless of single or mix inoculation treatments. After inoculating phosphobacteria, the invading capability of bacteria was stronger than actinomyces and fungi, and the quantity of bacteria was bigger than the tatter. Inoculation Y4 could improve the net photosynthetic rate, but the effect was gradually weak, while inoculation other suspension decreased the net photosynthetic rate. Inoculation Y4 could increase the content of total P. 【Conclusion】 The colonization quantity of phosphobacteria is decreasing with the increasing of introduced microorganism. Bacteria have more effect on the colonization of phosphobacteria. Inoculation Pseudomonas and Bacillus promote plant growth and development.

关 键 词：本溪山樱 根部 解磷细菌 定殖 

分 类 号：S662.5[农业科学—果树学]