烧伤小鼠早期P56与IFN mRNA表达变化及麻醉或KPV对其表达的影响  

Expressions of P56,IFN-α and IFN-β mRNAs in major organs of severe burn mice and effects of KPV and anaesthesia

作　　者：李淑蓉[1] 粟永萍[2] 邓正阳[2] 楼淑芬[2] 程天民[2] 

机构地区：[1]成都医学院病理学教研室,成都610083 [2]第三军医大学军事预防医学院防原医学教研室,全军复合伤研究所,创伤、烧伤与复合伤国家重点实验室,重庆400038

出　　处：《第三军医大学学报》2008年第5期367-369,共3页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划项目(“973”项目)(G1999054201)~~

摘　　要：目的探讨小鼠烧伤早期干扰素诱导蛋白P56、IFN-β、IFN-α mRNA在肺、肝、脾、肾组织中的表达,以及烧伤后给予麻醉剂或KPV对其表达的影响。方法选取健康昆明鼠40只,用随机排列表法分为4组:正常对照组、单纯烧伤组、烧伤+KPV组(简称KPV组)、烧伤+麻醉组(简称麻醉组),烧伤后3h取肝、肺、肾、脾组织,提取RNA,用两步法RT-PCR检测P56、IFN-β、IFN-αmRNA的表达。结果P56 mRNA在肝、脾、肺、肾组织中都有表达,且表达丰度较高。在肾组织中烧伤组和KPV组表达显著高于正常组(P<0.05),麻醉组P56 mRNA表达与正常组相似;IFN-β mRNA在肝组织中不表达,而在脾、肺、肾组织中均有表达,但表达量不高,在肺组织中烧伤组和麻醉组表达显著增加(P<0.05),KPV组表达略微降低;IFN-α mRNA在所有已测脏器中都有表达,在肾组织中烧伤组和KPV组较正常组表达显著增加(P<0.05),麻醉组增加不明显。结论烧伤后干扰素表达增加,诱导P56表达增加,说明P56可能与早期创伤应激不良有关。Objective To investigate the expressions of P56, IFN-β, IFN-α mRNAs and the effects of KPV, a COOH-terminal tripeptide sequence of alpha-melanocyte stimulating hormone [ alpha-MSH（ 11 - 13 ） ], and anaesthesia on the expressions in severe burn injured mice. Methods Eighty mice were randomly and equally divided into 4 groups： control, burn （TBS15% Ⅱ ）, burn ＋ KPV （injection of 40 μg per animal immediately after burn）, and burn ＋ anaesthesia （ injection of 0.4 mg/kg diazepam and 10 mg/kg ketamine immediately after burn）. All animals were sacrificed in 3 h after burn respectively. The expressions of P56, IFN-β, and IFN-α mRNAs in liver, spleen, lung and kidney tissues were detected with RT-PCR. Results P56 was expressed highly in liver, spleen, lung, and kidney in normal control group, and was obviously increased in kidney in burn group and burn ＋ KPV group, but that in burn ＋ anaesthesia group had no significant difference with that in normal control. IFN-β was not expressed in live and lightly expressed in spleen, lung and kidney, and its expression in lung was significantly increased in burn group and burn ＋ anaesthesia group, but mildly decreased in burn ＋ KPV group. IFN-α mRNA was expressed in all detected organs in normal control, and highly expressed in kidney of burn group and burn ＋ KPV group, but not in burn ＋ anaesthesia group. Conclusion These results indicate that the increased IFN expressing induce the up-regulation of P56, suggesting which may be involved in the disorder of early post trauma stress.

关 键 词：烧伤 干扰素诱导蛋白P56 IFN-Β IFN-Α 

分 类 号：R392.11[医药卫生—免疫学] R394.2[医药卫生—基础医学]