七氟烷和异氟烷对肝脏缺血再灌注损伤的影响  

Effect of Sevoflurane and Isoflurane on Liver against Ischemia-reperfusion Injury in Rats

作　　者：王旭东[1] 卢雅立[1] 王飞[2] 李艳萍[2] 

机构地区：[1]中山大学肿瘤防治中心麻醉科,广东广州510060 [2]中山大学附属第二医院麻醉科,广东广州510120

出　　处：《中山大学学报（医学科学版）》2008年第1期30-33,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科委科研基金资助项目(2005B30301001);广东省医学科学技术研究基金(A2005249)

摘　　要：【目的】探讨七氟烷和异氟烷预处理对大鼠肝脏缺血再灌注损伤(I/R)的保护作用及机制。【方法】96只SD大鼠随机分为假手术对照组(Sham)、肝脏缺血再灌注组(IR)、七氟烷预处理(Sev)和异氟烷预处理组(Iso),于肝脏缺血30min,再灌注30min、120min测各组大鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)的含量以及肝脏组织中谷胱甘肽(GSH)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。【结果】肝脏缺血再灌注后IR、Sev和Iso组血清ALT、AST和LDH含量均显著升高,肝组织SOD和GSH显著降低,而MDA明显升高,和Sham组相比有显著差异(P<0.01);Sev、Iso组和IR组比较,ALT、AST、LDH和MDA降低,而SOD和GSH显著升高(P<0.05或P<0.01),其中Sev组比Iso组效果更为明显(P<0.05或P<0.01)。【结论】七氟烷和异氟烷对肝脏缺血再灌注损伤有一定的保护作用,这可能与其抑制氧自由基生成,减少脂质过氧化有关。[Objective] To investigate the protective effect of sevoflurane and isoflurane on the liver against ischemia-reperfusion injury and the mechanisms involved. [Methods] Ninety-six SD rats were randomly divided into four groups： sham-operation group （Sham）, ischemia-reperfusion group （IR）, sevoflurane group （Sev） and isoflurane group （Iso）. Serum levels of alanine aminotransferase （ALT）, aspartate aminotransferase （AST） and lactate dehydrogenase （LDH） were determined after ischemia-reperfusion. Superoxide dismutase （SOD） activities, glutathione （GSH） and malondialdehyde （MDA） content in liver were measured. [Result] The activities of serum ALT, AST, and LDH in IR, Sev, and Iso groups were significantly higher than that in Sham group （P 〈 0.01） after ischemia-reperfusion. Compared with those in Sham group, SOD activities, GSH content in liver degraded and meanwhile the content of MDA increased in IR, Sev, and Iso groups after ischemia-reperfusion （P 〈 0.05 or P 〈 0.01）. The activities of ALT, AST, LDH and the content of MDA were lower than those in IR group （P 〈 0.05 or P 〈 0.01 ）. Meawhile the SOD activities and GSH content in liver were higher than those in IR group （P 〈 0.05 or P 〈 0.01 ）. [Conclusion] Both sevoflurane and isoflurane can protect liver from ischemia and reperfusion injury. The protective mechanisms maybe related to reduceing the release of oxyradical and inhibiting the lipid peroxidation.

关 键 词：七氟烷 异氟烷 肝脏 再灌注损伤 

分 类 号：R322.47[医药卫生—人体解剖和组织胚胎学]