哮喘患儿转录因子T-bet/GATA-3 mRNA比值及其与Th1/Th2平衡间的关系  

T-bet/GATA-3 mRNA Ratio as a Novel Measure of Th1/Th2 Balance in Asthmatic Children

作　　者：檀卫平[1] 李静[1] 夏焱[1] 吴葆菁[1] 麦贤弟[1] 李晓圆[1] 黄花荣[1] 黄绍良[1] 

机构地区：[1]中山大学附属第二医院儿科,广东广州510120

出　　处：《中山大学学报（医学科学版）》2008年第1期95-98,112,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省自然科学基金(031668A;6021301A)

摘　　要：【目的】研究转录因子T-bet/GATA-3在小儿哮喘发病以及Th1/Th2失衡中的作用。【方法】本实验拟通过半定量RT-PCR检测部分哮喘患儿外周血单个核细胞(PBMC)中T-bet、GATA-3 mRNA表达情况,同时采用三色荧光流式细胞仪检测哮喘患儿的Th1、Th2细胞数量,并对T-bet、GATA-3 mRNA表达水平与Th1、Th2细胞比率进行相关分析,了解T-bet/GATA-3对哮喘患儿的Th细胞分化的调节作用。【结果】哮喘患儿外周血淋巴细胞转录因子T-bet mRNA表达水平显著低于正常对照组,其中哮喘发作组显著低于缓解组(P<0.05);转录因子GATA-3 mRNA表达水平显著高于正常对照组,哮喘发作组又显著高于缓解组(P<0.05)。哮喘患儿外周血淋巴细胞亚群Th1百分率显著低于正常对照组,其中哮喘发作组又显著低于缓解组(P<0.05);Th2细胞百分率显著高于正常对照组,哮喘发作组又显著高于缓解组(P<0.05)。哮喘患儿T-bet mRNA表达量与Th1细胞比率呈正相关(r=0.731),与Th2细胞比率呈负相关(r=-0.685);GATA-3 mRNA表达量与Th1细胞比率呈负相关(r=-0.692),与Th2细胞比率呈正相关(r=0.717);T-bet/GATA-3 mRNA比值与Th1细胞比率呈正相关(r=0.793),与Th2细胞比率呈负相关(r=-0.639),与Th1/Th2比值呈正相关(r=0.801)。【结论】哮喘患儿T-bet/GATA-3 mRNA表达水平失调,与哮喘患儿Th1/Th2失衡密切相关,可作为衡量Th1/Th2平衡的新指标。[Objective] To explore the effect of T-bet and GATA-3 mRNA expression on profiles of type 1 and type 2 Th cells in asthmatic children. [Method] To detect the mRNA expression of T-bet and GATA-3 in the PBMC by semi-quantitative PCR and determine Thl and Th2 cell numbers by FACS in 38 asthmatic children, including acute attack group of 20 （age 3-13, 6.2 ± 2.9）; 18 of remission group （age 3-12, 6.1 ± 2.5）; and 20 healthy control children （age 3-12, 6.9 ± 2.7）. [Result] It was showed that T-bet mRNA in asthmatic children was lower than control group and lower in attack stage than in remission stage （0.14 ± 0.035 vs 0.21 ± 0,033 vs 0.28 ± 0.031, P 〈 0.05）. To opposite, GATA-3 mRNA was higher in asthmatic children than control group and higher in attack stage than in remission stage （0.49 ± 0.091 vs 0.44 ± 0.083 vs 0.37 ± 0.041, P 〈 0.05）. It was showed that Thl percentage was lower in asthmatic children than those of control group and lower in attack stage than those of remission stage （9.67 ± 3.80 vs 14.69 ± 3.16 vs 21.18 ± 3.45,P 〈 0.05）. Th2 percentage in asthmatic children was higher than that of control group and higher in attack stage than that of remission stage （20.76 ± 7.95 vs 14.28 ± 1.71 vs 4.1 ± 1.55,P 〈 0.05）. Spearman correlation analysis revealed that T-bet mRNA was positively correlated with Thl percentage （r=0.731） and negatively correlated with Th2 percentage （r=-0.685）. GATA3 mRNA level was negatively correlated with Thl percentage （r=-0.692） and positively correlated with Th2 percentage （r=0.717）. The T-bet/GATA-3 mRNA ratio was positively correlated with Thl percentage （r=0.793） and Th1/Th2 ratio （r= 0.801）, but negatively correlated with Th2 percentage （r=-0.639）. [Conclusion]The T-bet/GATA-3 mRNA expression is closely correlated with Th1/Th2 balance, and the T-bet/GATA-3 ratio may act as a new measure of the Th1/Th2 balance in asthmatic children.

关 键 词：哮喘 儿童 T-BET GATA-3 TH1 TH2 

分 类 号：R725.6[医药卫生—儿科]