人胎胃壁内氮能神经元发育的免疫组化观察  

An observation on the development of nitrergic neurons in the gastric wall of human fetus with immunohistochemical techniques

作　　者：顾倩[1] 郑兰荣[1] 邵金贵[1] 

机构地区：[1]皖南医学院,安徽省芜湖市241001

出　　处：《实用医学杂志》2008年第4期512-514,共3页The Journal of Practical Medicine

基　　金：2007年度高校省级自然科学研究项目基金资助项目(编号:KJ2007B019)

摘　　要：目的:探讨人胎胃壁氮能神经元的发育规律。方法:用免疫组织化学法对人胎发育早期胃壁氮能神经元的表达进行观察。结果:第3个月龄时,胃壁肌间神经丛、环肌层和黏膜下层内均可见胞体较小、淡染的氮能神经元,有的神经元有短小突起;在肌层和黏膜下层靠近环行肌一侧可见到氮能神经节。第4个月龄时,胃壁肌间神经丛中氮能神经元数目增多,有少数神经元胞体明显增大,突起伸长,一氧化氮合酶(NOS)阳性反应增强;多数神经元胞体较小,呈索状排列或散在分布;环行肌纤维之间出现稀疏分布的神经纤维,其走向与环行肌纤维平行。第5个月龄时,在肌束周围形成明显的神经纤维束;黏膜下层出现大量胞体小、染色淡、呈散在分布、形态各异的氮能神经元。结论:胃壁内NOS阳性神经元的发育与胃壁各层组织的发育密切相关。Objective To explore the rule of the development of nitrergic neurons in the gastric wall of human fetus. Methods The expression of nitrergic neurons in the gastric wall of human fetus was observed using immunohistochemical method. Results At the third month of gestation, some small nitrergic neurons could be seen in the intermuscular plexus, the circular muscle layer and the submucosa of the gastric wall, with weak dyeing, some of which had a short process. Some nitrergic nerve ganglions were observed in the muscle layer or in the submucosa along the side of the circular muscle layer. At the fourth month, the numbers of the nitrergic neurons were increased in the intermuscular nerve plexus, the bodies of a few nitrergic neuron were enlarged, the process lengthened, the positive reaction of nitric oxide synthase increased. The bodies of most nitrergic neuron were small, arranged as funicular form or scattered. A few nitrergic nerve fibers were distributed in the circular muscle layer, and moved paralleling to the muscle fibers. At the fifth month, the nerve fiber bundles were obviously formed surrounding the muscle bundles; At the same time, the bodies of most nitrergic neuron were small, the cytoplasm was slightly stained, a great diversity of the shapes of which were diffusively distributed in the submucosa. Conclusion The growth and development of nitrergic neurons is closely connected with the cells and tissues in the different layers within the gastric wall

关 键 词：氮能神经元 免疫组织化学 胃壁 人胎 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]