阿霉素肾病大鼠肾小管基质金属蛋白酶-2、金属蛋白酶抑制剂-2的免疫组织化学观察  

Expressions of MMP-2 and TIMP-2 in renal tubule in rats with adriamycin-induced nephropathy

作　　者：李广波[1] 林瑞霞[1] 杨青[1] 唐雪栋[1] 

机构地区：[1]温州医学院附属育英儿童医院肾内科,325027

出　　处：《实用医学杂志》2008年第4期522-525,共4页The Journal of Practical Medicine

基　　金：浙江省教育厅基金资助项目(编号:20061807)

摘　　要：目的:探讨基质金属蛋白酶-2(matrix metalloproteinases-2,MMP-2)和金属蛋白酶抑制剂-2(tissue inhibitor of metalloproteinase-2,TIMP-2)在阿霉素肾病大鼠肾小管中的作用。方法:采用大鼠尾静脉单剂量注射阿霉素7.5mg/kg建立阿霉素肾病模型,免疫组织化学检测正常对照组、肾病模型组肾组织MMP-2及TIMP-2的表达变化。结果:肾病模型组大鼠肾小管上皮细胞MMP-2和TIMP-2的表达明显下降,与正常对照组相比差异显著(P<0.01)。结论:MMP-2和TIMP-2在阿霉素肾病的发病过程中可能起重要的作用。Objective To explore the roles of MMP-2 and TIMP-2 in the pathogenesis of adriamycin-induced nephropathy in rats. Methods The rat model of nephropathy was established by an injection of adriamycin at a dose of 7.5 mg/kg via the tail vein. The expressions of MMP-2 and TIMP-2 in the renal epithelial cells in the normal control tubules and the diseased tubules were examined by immunohistochemistry. Results The expressions of MMP-2 and TIMP-2 in the diseased tubules were significantly declined than those in the normal tubules （P 〈 0. 01 ）. Conclusion MMP-2 and TIMP-2 may play an important role in the pathogenesis of adriamycin-induced nephropathy.

关 键 词：肾病 明胶酶A 纤维化 组织金属蛋白酶抑制剂 

分 类 号：R692[医药卫生—泌尿科学]