ns脉冲电场发生器的研制及应用  

Development and Application of Nanosecond Pulsed Electric Fields Generator

作　　者：米彦[1] 莫登斌[1] 姚陈果[1] 李成祥[1] 孙才新[1] 唐丽灵[2] 刘欢[2] 

机构地区：[1]重庆大学输配电装备及系统安全与新技术国家重点实验室,重庆400044 [2]重庆大学生物工程学院,重庆400044

出　　处：《高电压技术》2008年第2期368-372,共5页High Voltage Engineering

基　　金：国家自然科学基金(50637020,50407007);高等学校博士学科点专项科研基金(20050611020);重庆市科委重大专项(2005AA5008-3)~~

摘　　要：为了研究ns脉冲电场诱导肿瘤细胞的凋亡效应,结合开关电源技术和脉冲功率技术,研制了一套多参数大范围独立可调的ns脉冲电场发生器。该发生器由高压直流电源、脉冲形成电路和辅助电路3部分组成,输出的脉冲幅值0～1.2kV内连续可调,重复频率1Hz～10kHz内连续可调,脉冲宽度分100、200、600和1000ns4级可调,脉冲个数可以任意预置,上升时间<30ns,并具有显示和保护功能。实验室调试和医学细胞实验结果表明,该发生器调节方便、性能稳定,能够有效诱导人肝癌细胞凋亡,为ns脉冲电场诱导肿瘤细胞凋亡的机理和阈值参数选择规律的深入研究奠定了基础。In order to research apoptosis effects of cancer cell induced by nanosecond pulsed electric fields （nsPEF）, on the basis of combination of switching power supply technology with pulsed power technology, this paper developed a nsPEF generator whose output multiple parameters could be adjusted in a wide range and independently. The generator was composed of a high-voltage direct current power supply, a pulse forming circuit and an assistant circuit. The amplitude of output pulse could be adjusted continually from 0 V to 1.2 kV by means of adjusting the driving signal in high-voltage direct current power supply. The repeat rate of output pulse could be adjusted continually from 1 Hz to 1 kHz by means of adjusting the control circuit in assistant circuit. The width of output pulse could be adjusted to lOOns, 200 ns, 600 ns and 1 000 ns by means of switching to different energy storing capacitor in pulse forming circuit. The number of output pulse could be preset to any value and the rise time of output pulse was less than 30 ns. All above-mentioned parameters could be displayed via LED. Over-voltage, over-current and short circuit protections were also available. Debugging results in laboratory and medical cell experiment results show that nsPEF generator can be adjusted conveniently and operated stably. Furthermore, nsPEF can induce apoptosis of human liver cancer cell effectively. In conclusion, this nsPEF generator is competent for research of mechanism and optimal parameters for cancer cell apoptosis induction.

关 键 词：ns脉冲电场 发生器 工作原理 应用 人肝癌细胞 凋亡 流式细胞术 

分 类 号：TM89[电气工程—高电压与绝缘技术]