子宫内膜局部的瘦素信号传导系统及其调控  

Endometrial Partial Leptin Signal Transduction System and Its Regulation

作　　者：张跃辉[1] 吴效科[1] 曲军卫[2] 王勇[2] 侯丽辉[1] Erkkola Risto Strauss Jerome 匡海学[5] 

机构地区：[1]黑龙江中医药大学附属第一医院妇产科,哈尔滨150040 [2]南京大学医学院,南京210009 [3]芬兰图尔库大学中心医院妇产科 [4]美国弗吉尼亚邦联大学医学院 [5]黑龙江中医药大学,哈尔滨150040

出　　处：《生殖与避孕》2008年第2期74-79,共6页Reproduction and Contraception

基　　金：中国-芬兰科学技术合作基金(2004-2006第11次会议);国家自然科学基金(30572404);黑龙江省自然科学基金重点项目(ZJY0506-01)

摘　　要：目的:探讨瘦素及瘦素信号传导系统中各关键信号蛋白在子宫内膜的表达及其调控。方法:健康雌性B6.Cg-m+/+Leprdb小鼠(实验组)和其同窝出生的雌性野生型C57BL/6J小鼠(对照组)各10只,于16周龄的动情间期进行瘦素刺激实验,用免疫组化法检测瘦素及瘦素信号传导系统中各关键信号蛋白在子宫内膜的表达情况。结果:①16周龄时与对照组比,实验组较其体重增加约1倍(P<0.01),性腺周围脂肪垫重量增加约10倍(P<0.01);血葡萄糖、低密度脂蛋白胆固醇(LDL-C)和胰岛素水平增加2-3倍(P<0.01);子宫重量及E2、P、FSH、LH水平显著下降(P<0.05)。阴道涂片发现对照组均出现正常的动情周期,而实验组则始终处于动情间期。②免疫组化研究显示,瘦素信号传导系统的各关键信号蛋白在对照组子宫内膜间质中均有表达,但在子宫瘦素受体缺失组则JAK2为阴性表达,stat3的磷酸化过程也受阻。结论:①瘦素对子宫内膜存在影响。②体内瘦素受体缺失引起机体的高糖脂血症导致了子宫的脂质化,同时子宫局部的瘦素信号传导缺失,均可能参与生殖功能障碍。Objective： To investigate the expression and regulation of key signaling protein of the leptin and leptin signal transduction systems in endometrium. Methods： Healthy female B6.Cg-m ＋/＋ Leprdb mice were collected as experimental group （n=10）. After leptin stimulation in estrus interval, the levels of glucose and lipid metabolism and reproductive hormones were detected. Immunohistochemical method was used to detect the expression of the key signaling protein of leptin and leptin signal system in endometrium. Results： 1） At 16-week old, the mice had doublet weight in experimental group than in the control （P 〈0.01）, and gonadal fat weight was increased about 10-folds （P 〈0.01）; blood glucose, low density of lipoprotein cholesterol （LDL-C） and insulin levels were increased 2-3 folds （P 〈0.01）, uterine weight and E2, P, FSH, LH level were significantly decreased （P 〈0.05）. Vaginal smears were found the normal estrous cycle in the control, and were always estrus interval in experimental group. 2） Immunohistochemical study showed that all of the key signaling protein of the leptin signal transduction systems were expressed in endometrial stromal of control gruoup mice, but JAK2 had negative expression and phosphorylation process of stat3 was obstructed. Conclusion： 1） Leptin can impact on the uterus. 2） Leptin receptor missing can lead to high glycolipids and hyperlipidemia, further more, all of these lead the uterus to lipid, and uterine partial loss of leptin signal transduction, are likely to be involved in reproductive dysfunction.

关 键 词：子宫 瘦素 瘦素受体 信号传导 

分 类 号：R711[医药卫生—妇产科学]