全雌性黄瓜中3个肌动蛋白基因片段的克隆和表达分析  

Cloning and Characterization of Three Actin Genes in Gynoecious Line Cucumber (Cucumis sativus L.)

作　　者：徐纪明[1] 向太和[1] 

机构地区：[1]杭州师范大学生命与环境科学学院,杭州310036

出　　处：《细胞生物学杂志》2008年第1期125-130,共6页Chinese Journal of Cell Biology

基　　金：浙江省自然科学基金(No.Y304083);杭州市科技创新基金(No.20070232H07);杭州市"131"人才基金资助项目~~

摘　　要：通过1次PCR扩增从全雌性黄瓜中同时获得了长度分别为1186bp、955bp和870bp的3个肌动蛋白基因片段Act1、Act2和Act3(GenBank登记号:DQ115881、DQ115882和DQ115883)。序列分析表明,3个肌动蛋白基因片段与GenBank中收录的肌动蛋白基因序列高度同源,与GenBank中收录的全长肌动蛋白基因(如AF386514.1、AF059484.1和AB010922.1等)的第2、第3个外显子和第2个内含子相对应。Act1中1￣580bp、984￣1186bp,Act2中1￣580bp、753￣955bp,Act3中1￣580bp、668￣870bp为外显子编码区,其余为内含子序列,3个基因内含子的两端序列均符合典型的"GT-AG"规则。RT-PCR分析表明,Act1仅在根中表达,Act2在根和雌花中表达,而Act3在所有检测的器官(包括根、茎、叶、卷须、雌花和幼果)中都表达,提示黄瓜根的生长发育需要多种肌动蛋白基因的参与,而Act2和Act3可能与黄瓜雌性系的形成发育有关。Three actin genes with length of 1 186 bp, 955 bp and 870 bp named as Act1, Act2 and Act3 （GenBank accession number： DQ115881, DQ115882 and DQ 115883） from gynoecious cucumber line were cloned in one-step PCR reaction. The cloned genes share high similarity to the 2th exon, 3th exon and 2th intron of other actin gene in the GenBank （AF386514.1, AF059484.1 and AB010922.1 etc.）. The analysis of RT-PCR revealed that Actl expressed in root only, the Act2 could be detected in root and female flower, while the Act3 expressed in all tested organs. The cloned genes enriched the actin gene family and could be employed to study molecular and physiological mechanism of actin gene. In addition, the result indicates that the origin and evolution of plant actin genes were highly homologous and conservative respectively.

关 键 词：黄瓜 肌动蛋白基因 克隆 表达 

分 类 号：Q943.2[生物学—植物学]