高效液相色谱法测定水性涂料中的酚类防霉剂  

Determination of chlorophenol preservatives in coatings by high performance liquid chromatography

作　　者：牛增元[1] 包艳[2] 叶曦雯[1] 汤志旭[1] 罗忻[1] 

机构地区：[1]山东出入境检验检疫局,青岛266002 [2]中国科学院青岛生物能源与过程研究所,青岛266000

出　　处：《分析试验室》2008年第3期48-51,共4页Chinese Journal of Analysis Laboratory

基　　金：质检公益性行业科研专项(10-66)经费资助

摘　　要：建立了简便、准确的高效液相色谱法检测涂料样品中五氯苯酚（PCP）、四氯苯酚（TeCP）和邻苯基苯酚（OPP）。选用C18柱为分离柱,以甲醇-10 mmol/LNH4Ac为流动相进行梯度洗脱,二极管阵列检测器检测,检测波长为220 nm。在该色谱条件下,PCP、TeCP、OPP分析线性范围分别为0.02-200 mg/L,0.02-200 mg/L,0.04-200 mg/L;最低检测浓度分别为0.01、0.01、0.02 mg/L,分别相当于涂料中的质量分数为5.0×10^-4%,5.0×10^-4%,1.0×10^-3%;方法加标回收率为96.6%-103.6%,相对标准偏差为0.98-4.3%（n=7）。本法能满足涂料中PCP、TeCP、OPP的分析测定。An HPLC analytical method was developed for the simultaneous separation and determination of pentachlorophenol （PCP）, tetrachlorophenol （TeCP） and orthophenylphenol （OPP） in coatings. Experiments were performed on Venusil-XBP C18（4.6 mm i.d. ×250 mm, 5 μm） column, with CH3OH and NH4Ac as mobile phase using gradient elution at a flow-rate of 0.7 mL/min and detected with a photodiode array detector at 220 nm. The linear range of PCP, TeCP and OPP were 0.02 - 200 mg/L, 0.02 - 200 mg/L and 0.04 - 200 mg/L respectively. The recoveries of this method in the range of 96.6% - 103.7%, with RSD below 4.3% （n =7）. This method can be applied to the determination of PCP, TeCP and OPP in coatings with satisfactory results

关 键 词：涂料 高效液相色谱 五氯苯酚 四氯苯酚 邻苯基苯酚 

分 类 号：O657.7[理学—分析化学]