胰岛素样生长因子结合蛋白6在维甲酸诱导的大鼠马蹄内翻足畸形中的表达研究  

Expression of IGFBP-6 in rats with retinoic acid-induced congenital talipes equinovarus

作　　者：郭磊[1] 赵玉岩[2] 张世亮[1] 朱世博[1] 刘魁[1] 

机构地区：[1]中国医科大学附属第一医院骨科,沈阳110001 [2]中国医科大学附属第一医院内分泌科,沈阳110001

出　　处：《中华小儿外科杂志》2008年第2期118-121,共4页Chinese Journal of Pediatric Surgery

基　　金：国家自然科学基金（编号：30500414）,辽宁省教育厅高等学校科学研究项目（编号：05L508,20061010）

摘　　要：目的探讨大鼠先天性马蹄内翻足（CCF）软骨组织内胰岛素样生长因子结合蛋白6（IGFBP-6）的表达规律。方法应用生物学活性物质全反式维甲酸（ATRA）构建大鼠CCF动物模型；光镜和透射电镜观测大鼠的足部软骨组织病理学改变；RT-PCR及Western印迹杂交检测胎鼠足部软骨组织中IGFBP-6mRNA及蛋白质的表达水平。结果120mg／kg的ATRA诱导实验组35％的胎鼠出现CCF畸形，其他畸形包括：内翻足、小脑畸形、腭裂、无尾畸形等；在骨骼畸形胎鼠的足部距骨内软骨发生中心带缩小，软骨细胞数量减少，软骨细胞核内粗面内质网扩张，线粒体嵴紊乱；正常胎鼠软骨组织内IGFBP-6基因呈低水平表达状态，而骨骼畸形胎鼠IGFBP-6的mRNA和蛋白质表达则明显升高300％和197％（P〈0．05）。结论正常大鼠软骨组织内IGFBP-6基因有低表达。CCF大鼠软骨组织内IGFBP-6的高表达可能与骨骼发育畸形的发生密切相关。Objective To investigate the gene expression of insulin- like growth factor binding protein 6 （IGFBP - 6） in fetal rat with congenital talipes equinovarus （CCF）. Methods The fetal rat models with CCF were constructed treated with all trans retinoic acid （ATRA） on pregnant day 10. The histopathologic characteristics of cartilaginous tissue in fetal foot with skeletal deformity was detected with light microscope and transmission electron microscope; The IGFBP- 6 mRNA and protein level in cartilaginous tissue were detected by Reverse Transcription Polymerase Chain Reaction and Western Blotting. Results ATRA（120mg/kg）induced single CCF or coalescent developmental skeleton defect in fetal rat （35%）, such as cross-foot, cleft spine, cleft palate, rumpless and so on. The chondrogenesis center of tarsal bones and quantity of chondrocyte were diminished. There were expanding rough endoplasmic reticulum, degradation of nuclear matrix, and disorder of mitochondria cristae in cell nucleus of chondrocyte. The expression of IGFBP-6 mRNA and protein were increased 300% and 197% in skeleton defect rat,compared with normal rat （P〈0. 05）. Conclusions There was low gene expression of IGFBP-6 in normal rat cartilaginous tissue and higher expression in CCF. The higher gene expression of IGFBP-6 may be induced the developmental skeleton defect in CCF rat.

关 键 词：畸形足 先天性 胰岛素样生长因子结合蛋白质6 维甲酸 

分 类 号：R726.8[医药卫生—儿科]