聚焦超声对慢性湿疹模型豚鼠皮肤淋巴细胞和角质形成细胞凋亡的影响  

Effect of focused ultrasound on the apoptosis of lymphocytes and keratinocytes in guinea pig model of chronic eczema

作　　者：祝青[1] 刁庆春[2] 白晋[1] 王智彪[1] 

机构地区：[1]重庆医科大学生物医学工程系、医学超声工程研究所,重庆400016 [2]重庆市第一人民医院皮肤科,重庆400011

出　　处：《中国医学影像技术》2008年第2期167-170,共4页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学杰出青年基金(30325027);超声医疗国家工程研究中心资助

摘　　要：目的探讨聚焦超声诱导慢性湿疹模型豚鼠皮肤淋巴细胞和角质形成细胞凋亡的作用及其机制。方法健康豚鼠40只,每只背部剃毛2处,应用2,4-二硝基氯苯为抗原,小剂量多次刺激豚鼠皮肤制备慢性湿疹模型。将模型随机分为1W组和2W组,自身空白对照。辐照后在24、48h采用TUNEL法和免疫组化法观察2W组、1W组和对照组细胞凋亡及Bcl-2和Bax蛋白表达情况。结果辐照组的皮肤淋巴细胞和角质形成细胞凋亡率和Bax蛋白表达高于对照组,2W组高于1W组,48h高于24h。辐照组Bcl-2蛋白表达低于对照组。Bcl-2蛋白表达与凋亡细胞率呈负相关,Bax与凋亡细胞率呈正相关。结论聚焦超声辐照豚鼠慢性湿疹模型可明显提高Bax的表达,抑制Bcl-2的表达,进而促进慢性湿疹动物模型皮肤淋巴细胞和角质形成细胞的凋亡。Objective To study apoptosis expression and its mechanism in lymphocytes and keratinocytes after focused ultrasound on guinea pig model of chronic eczema. Methods A total of 40 guinea pigs were shaved at two sites on the back.The eczema models were prepared by using 2, 4-dinitrochlorobenzene as an antigen to stimulate the shaved sites repeatedly and divided into 1W group and 2W group at random. The focused ultrasound treated one shaved site and the opposite site as the control without irradiation. Each group were observed at 24 h and 48 h after ultrasound irradiation. TUNEL was used to detect the distribution of apoptosis cells. The immunohistochemical method was used to detect the distribution of Bcl-2 and Bax protein in the skin tissues. Results Both the average percentage of apoptosis cells and Bax protein were higher in the treatment groups than in the control groups. The significant differentiation in Bcl-2 protein between the two groups was also observed.There was a positive correlated signification between apoptosis cells and Bax,and a negative correlation between apoptosis cells and Bcl-2. Conclusion Focused ultrasound can significantly induce the lymphocytes and keratinocytes of guinea pig model of chronic eczema to apoptosis and modulate the expression of the apoptosis related genes Bcl-2 and Bax.

关 键 词：超声 高强度聚焦的 豚鼠 湿疹 凋亡 BCL-2 BAX 

分 类 号：R815[医药卫生—放射医学]