Gatifloxacin-铕荧光探针测定DNA  

Determination of DNA with Gatifloxacin(GFTX)-Eu^(3+) as Complex Fluorescent Probe

作　　者：敖登高娃[1] 斯琴 赵永亮[1] 

机构地区：[1]内蒙古大学化学化工学院,内蒙古呼和浩特010021

出　　处：《中国稀土学报》2008年第1期24-27,共4页Journal of the Chinese Society of Rare Earths

基　　金：国家自然科学基金(20461002);内蒙古自然科学基金(200607010205);内蒙古自治区高等学校研究项目(NJ05040)

摘　　要：在pH 7.0±0.1的Tris-HCl缓冲溶液中,Eu3+与加替沙星形成的络合物受270 nm紫外光激发发出Eu3+的特征荧光峰,加入脱氧核糖核酸(DNA)能大大增强体系的荧光强度,由此建立GFLX-Eu3+络合物探针测定核酸的方法。与GFLX-Eu3+二元络合物相比,GFLX-Eu3+-DNA三元体系荧光强度显著增强,且最大吸收波长紫移5 nm,表明形成三元络合物。研究了反应的最佳条件,并初步探讨了GFLX-Eu3+络合物探针和DNA结合机制。The europium（Ⅲ） ion could be to form a complex with gatifloxacin in pH = 7.0 - 0.1 Tris-HC1 buffer solution, then emitted the characteristic fluorescence of europium（ Ⅲ ） ion. While the DNA was added, the fluorescence intensity of the syetem was greatly increased. Based on this principle and special property, a sensitive method so called probe method for the determination of DNA was established. It was found that compared with the binary complex of GFLX-Eu^3＋ the ternary systems of GFLX-Eu^3＋-DNA had larger fluorescence intensity and their maximal absorptions are blue-shifted about 5 nm, indicated that an ternary complex was found. The optimum reaction conditions were discussed and the mechanism for the ternary complex formation was preliminary proposed.

关 键 词：核酸 加替沙星 铕 荧光光谱法 稀土 

分 类 号：O482.3[理学—固体物理]