氯化钆对糖尿病小鼠模型的降糖作用  

Gadolinium Chloride Down-Regulated Glucose Level of Type I Diabetic Mice

作　　者：文锦华[1] 陈燊[2] 孙莹[1] 吴永华[1] 王宏[1] 洪天配[2] 李丽英[1] 李凌松[1] 

机构地区：[1]北京大学干细胞研究中心,北京大学医学部基础医学院细胞生物学系,北京100083 [2]北京大学第三医院内分泌科,北京100083

出　　处：《中国稀土学报》2008年第1期108-111,共4页Journal of the Chinese Society of Rare Earths

基　　金：北京自然科学基金重大项目(D07050701350704);973项目(2006CB943603,2006CB503905)资助

摘　　要：在离体培养人胰腺前体细胞时在培养基中加入GdCl3,观察发现GdCl3能够使人胰腺前体细胞聚集,在诱导因子共同存在下,进一步诱导人胰腺前体细胞成为胰岛样结构。用STZ诱导小鼠构建I型糖尿病小鼠模型。当每4 d给I型糖尿病小鼠腹腔注射GdCl3溶液,25 d后观察到小鼠的空腹血糖值明显降低,IPGTT试验结果显示,小鼠糖代谢得到明显改善。结果表明,可能的机制为:GdCl3通过促进胰腺干细胞分化成为成熟的胰岛素分泌细胞,增加内分泌细胞的数量,从而促进受损胰腺的再生与修复,起到降低糖尿病小鼠血糖水平的作用。The effects of gadolinium chloride on the human pancreatic stem cells in culture and on the regulation of blood glucose levels of mice were investigated. The cultured pancreatic stem cells were aggregated to islet-like clusters at the present of 1.0 mmol· L^-1 GdCl3. When a dose of 10 mg·kg^-1 b.w. GdCl3 were given to the type I diabetic injection, the levels of mice with intraperitoneal fasting glucose levels and blood glucose levels after IPGTT were measured. The results demonstrated that the treatment with the dose of 10 mg · kg^-1 b. w. GdCl3 down-regulated the blood glucose levels singnificantly. The results suggested that GdCl3 could induce the differentiation of pancreatic stem cells and maturation of β cells of pancreas in diabetic mice.

关 键 词：GdCl3 胰腺前体细胞 糖尿病小鼠模型 IPGTT 稀土 

分 类 号：Q254[生物学—细胞生物学] R587.1[医药卫生—内分泌]