HPLC法同时测定大鼠心脏中清脑宣窍方三种成分栀子苷、人参皂苷Rg_1、人参皂苷Rb_1的含量  

Three constituents of Qingnaoxuanqiao Formula,geniposide,ginsenoside Rg_1 and Rb_1,in rat heart determined quantitatively by HPLC

作　　者：周威[1] 石任兵[1] 孙建宁[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《北京中医药大学学报》2008年第2期112-115,121,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：高等学校科技创新工程重大项目培育资金项目(No.v200611)

摘　　要：目的建立大鼠心脏中清脑宣窍方有效部位中栀子苷、人参皂苷Rg1、Rb1的HPLC浓度测定方法。方法内脏样品匀浆、甲醇提取、离心后水溶,用C18固相柱萃取,以亥茅酚苷为内标,选用色谱柱：Agilent Eclipse XDB-C18（4.6 mm×250 mm,5μm）,Eclipse XDB-C18保护柱。流动相为乙腈-酸水二元梯度洗脱,柱温为30℃,流速为1.0 mL/min,检测波长为203 nm。结果各成分的最低检测浓度（S/N〉3）在0.16-1.1 mg/L之间,平均回收率均在90%以上,日内、日间精密度及稳定性的RSD均小于10%。结论该方法简便、准确,可用于心脏组织中清脑宣窍方有效部位栀子苷、人参皂苷Rg1、Rb1三种成分定量分析。Objective To establish a method for determinating the concentrations in rat heart of geniposide, ginsenoside Rg1 and ginsenoside Rb1 by HPLC in the effective fraction of Qingnaoxuanqiao Formula. Methods The rat heart samples were smashed into plasm, extracted by MeOH and dissolved by water after centrifuging. A column of C18 solid phase was used for the extraction and sec-oglucosylhamaudol was taken as an internal standard. The procedure was carried out on a chromatography column of Agilent Eclipse XDB-C18 （4.6 mm × 250 mm, 5μm） and a protective column of Eclipse XDB- C18. The mobile phase was a binary gradient elution of ethane nitrile to acid water and the column temperature was 30℃. The flow rate was 1.0 mL/min and the detection wavelength was 203 nm. Results The lowest testing concentration of each constituent （ S/N 〉 3 ） was from 0.16 to 1.1 mg/L. The average recovery was above 90 %. RSDs of accuracy and stability in intra-day and inter-day were all below 10 %. Conclusion The method is easy and accurate, which can be used in the quantitative analysis of 3 constituents in the effective fraction of Qingnaoxuanqiao formula in the heart tissue.

关 键 词：清脑宣窍方 栀子苷 人参皂苷Rg1 人参皂苷RB1 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]