北方农牧交错带林地、耕地和草地土壤微生物群落结构特征的PLFA分析  

APPLICATION OF PLFA ANALYSIS IN DETERMINATION OF SOIL MICROBIAL COMMUNITY STRUCTURE IN WOODLAND,CROPLAND AND GRASSLAND IN FARMLAND-PASTURE INTERLEAVING ZONE OF NORTH CHINA

作　　者：文倩[1] 林启美[1] 赵小蓉[1] 李贵桐[1] 赵沛一 

机构地区：[1]中国农业大学资源与环境学院 [2]内蒙古自治区农牧科学院,呼和浩特010031

出　　处：《土壤学报》2008年第2期321-327,共7页Acta Pedologica Sinica

基　　金：国家高技术研究发展计划(863计划)项目(2002AA2Z4221);国家“十五”科技攻关计划项目(2001BA508B12)资助

摘　　要：从内蒙古武川县采集了林地、雨养耕地、灌溉耕地和草地等4个不同利用方式的土壤，研究了土壤微生物磷酯脂肪酸的分布特征。结果表明，土壤总脂肪酸含量19．82～47．28ng g^-1，以指示G^-细菌的单烯脂肪酸和指示G^＋细菌的支链脂肪酸为主，其含量分别占脂肪酸总量的29％～37％和15％～20％，灌溉耕地和草地土壤明显高于林地和雨养耕地土壤，差异最大接近3倍。主成分分析表明，第一主成分（PCI）主要包括14：03OH、cy19：0、12：0、br17：0和18：2009，12等直链及支链脂肪酸；第二主成分（PC2）包括16：0、16：10ω11、18：1ω9、19：0和a15：0等不饱和及支链脂肪酸；灌溉耕地、雨养耕地、草地土壤有相似的微生物群落结构，与林地土壤有明显差异。Soil samples were collected from woodland, rain-fed cropland, irrigated cropland and grassland in Wuchuan County, Inner Mongolia in early May of 2002 in the study on distribution characteristics of soil microbial phospholipid fatty acids （PLFAs）. Soil microbial biomass carbon （MBC）, PLFAs and some soil basic properties were determined. Results showed that total PLFAs ranged from 19.82 ng g^-1to 47.28 ng g^-1, and monounsaturated fatty acids with G^- bacteria as indicator accounted for 29% - 37% and branch fatty acids with G^＋ bacteria for 15% - 20% of the total PLFAs. Irrigated cropland and grassland soils were significantly higher or nearly 3 times than rain-fed cropland and woodland soils in total PLFAs. Principal component analysis （PCA） indicated that PC1 consisted mainly of fatty acids of 14：0 3OH, cy19：0, 12：0, br17：0 and 18：2ω9,12, and PC2 mainly of 16：0, 16：1ω11, 18：1ω9, 19：0 and a15：0. The irrigated cropland, rain-fed cropland and grassland were quite similar in microbial community structure, but significantly different from woodland soil.

关 键 词：土壤微生物 磷脂脂肪酸 土地利用方式 半干旱地区 

分 类 号：S154.36[农业科学—土壤学]