槲皮素对人类乳腺癌细胞增殖的影响  

Effect of Quercetin on Proliferation of Human Breast Carcinoma Cells

作　　者：沈丽霞[1] 许惠玉[2] 赵丕文[1] 牛建昭[1] 王继峰[1] 

机构地区：[1]北京中医药大学细胞生化实验室,北京100029 [2]河北北方学院药理教研室,河北张家口075000

出　　处：《中国中医药信息杂志》2008年第3期30-32,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金资助(30371775;30510403202;30540420032);教育部长江学者和创新团队发展计划资助(IRT0413);教育部博士点科研基金资助(20050026012)

摘　　要：目的观察槲皮素(Quercetin,Que)对人类乳腺癌细胞株增殖的影响。方法采用MTT法测定槲皮素对雌激素依赖性乳腺癌细胞MCF-7和非雌激素依赖性乳腺癌细胞MDA-MB231细胞增殖作用,并以雌激素受体拮抗剂ICI182、780为工具药来评价槲皮素发挥雌激素样作用与雌激素受体(ER)的关系,采用流式细胞术对MCF-7细胞的增殖情况进行分析。结果与溶剂对照组相比较,Que(10～50μmol/L)能显著促进MCF-7细胞的增殖,而抑制ER阴性MDA-MB231细胞,并将MCF-7细胞周期由G1期向S期推进,促进DNA合成,提高细胞分裂增殖指数,且Que促进MCF-7细胞增殖作用被ER拮抗剂所拮抗。结论Que具有雌激素活性,此作用是通过ER介导的。Objective To investigate the effects of quercetin on the proliferation of mammary cancer cell in vitro. Methods Effects of quercetin on the cell proliferation was tested in ER-positive MCF-7 ceil and ER-negative MDA-MB231 ceil by MTT measurement. And to evaluate the estrogen-like effect of quercetin and its relation with the estrogen-receptor by pure estrogen receptor antagonist ICI 182, 780 as a tool. Cell cycle was examined by flow cytometry. Result Quercetin （10-50μmol/L） stimulated proliferation of ER-positive MCF-7 cell compared with solvent control, whereas ER-negative MDA-MB231 cell proliferation effect was inhibited, and the cell cycle was impulsed from G1 to S, DNA synthesizing was inhanced, PI was also increased. The above function on boosting MCF-7 ceil proliferation can be inhibited by adding estrogen receptor antagonism IC1182, 780, Conclusion Quercetin has the estrogen-like activities through the estrogen response pathway.

关 键 词：植物雌激素 槲皮素 雌激素受体 细胞增殖 MCF-7 MDA-MB231 

分 类 号：R285.5[医药卫生—中药学]